微流控芯片技术在精子优选中的研究进展

精子优选是体外受精( in vitro fertilization, IVF) 的关键步骤,对于提高人类辅助生殖(assisted reproductive technology,ART)成功率至关重要。研究证实,目前精子常规处理方法中的离心等过程可能造成精子活性氧(reactive oxygen species,ROS)产生增加,并损伤精子DNA。而自然受精过程中,精子需要穿过一系列解剖屏障,通过“微通道”进行迁移。以小型化和自动化为特征的微流控芯片技术恰能模拟精子在女性生殖管道中通过“微通道”自然迁移这一过程,从原精中优选获得高质量的精子,这对于提高IVF效率和改善妊娠结局具有很大的临床价值。本文对微流控芯片技术在精子优选中的研究进展进行综述,并对其应用前景进行了展望。

Research progress of microfluidic chip technology in sperm optimization

Sperm optimization is the key to in vitro fertilization (IVF) and is is extremely important to improve the success rate of assisted reproductive technology (ART). Studies have confirmed that processes such as centrifugation in the current conventional sperm treatment methods may increase reactive oxygen species (ROS) and damage sperm DNA. During the process of natural fertilization, sperm need to pass through a series of anatomical barriers and migrate through "microchannels". The microfluidic chip technology, characterized by miniaturization and automation, can simulate the process of natural migration of sperm through "microchannels" in the female reproductive tract, and optimally obtain high-quality sperm from the original semen, which is important for improving IVF efficiency and improving pregnancy outcomes. ThisSreview focuses onStheSrecent advancesSin sperm optimization implementedSinSmicrofluidicSchips and provide an outlook of its clinical application.