| 高效凝胶渗透色谱法测定茶叶糖蛋白含量研究 |
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| 引用本文： | 聂少平,谢明勇,王远兴. 高效凝胶渗透色谱法测定茶叶糖蛋白含量研究[J]. 现代中药研究与实践, 2004, 18(Z1): 40-42 |
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| 作者姓名： | 聂少平 谢明勇 王远兴 |
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| 作者单位： | 南昌大学食品科学教育部重点实验室,江西,南昌,330047 |
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| 基金项目： | 国家自然科学基金资助项目(批准号:20462005) |
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| 摘 要： | 用高效凝胶渗透色谱法(HPGPC)鉴定了茶叶糖蛋白(TGC)的纯度,并以TGC纯品作为标准品,以UltrahydrogelTM 500(7.8×300 mm)为固定相,0.6 mL／min蒸馏水为流动相,折光示差检测,对多种茶叶中TGC含量进行了测定,得回归方程为:Y=0.250 3×10-6-0.008 7,r=0.999 7,其浓度在0.604~6.04mg·mL-1范围内呈良好的线性关系。
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| 关 键 词： | 茶叶糖蛋白 高效凝胶渗透色谱法 含量测定 |
| 文章编号： | 1004-2199(2004)S0-0040-03 |
Determination of the Contents of Tea Glycoprotein by High Performance Gel Permeation Chromatography (HPGPC) |
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| Nie Shao-ping,Xie Ming-yong,Wang Yuan-xing. Determination of the Contents of Tea Glycoprotein by High Performance Gel Permeation Chromatography (HPGPC)[J]. Research and Practice on Chinese Medicines, 2004, 18(Z1): 40-42 |
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| Authors: | Nie Shao-ping Xie Ming-yong Wang Yuan-xing |
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| Abstract: | The purity of tea glycoprotein (TGC) was determined by high performance gel permeation chromatography (HPGPC). Then, this TGC was used as the standard substance for the determination of TGCs in various teas. The chromatographic conditions were with UltrahydrogelTM 500(7. 8×300 mm) column as stationary phase and the distilled water as the mobile phase at a flow rate of 0. 6 mL·min 1 at 35 C and the refractive index (RI) detector. The calibration curve was linear over the concentration range of 0. 604-6. 04 mg·mL-1 for TGC. The correlation coefficient was r = 0. 9997. The determination method of TGC developed in this paper was found to be simple, accurate and reliable. |
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| Keywords: | Tea glycoprotein (TGC) High performance gel permeation chromatography (HPGPC) Determination |
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