注射大剂量促黄体激素释放激素类似物后受孕大鼠垂体促性腺激素细胞的免疫组织化学变化

本文用特异的抗鼠促黄体激素(抗LH)、抗鼠卵泡刺激素(抗FSH)和抗人绒毛膜促性腺激素(hCG)血清,以PAP免疫组织化学方法,观察注射大剂量促黄体激素释放激素类似物(LHRH-A)后受孕大鼠垂体促性腺激素细胞的变化。注射后24 h,促性腺激素细胞的免疫组织化学反应明显减弱;注射后48 h,促性腺激素细胞的免疫组织化学反应开始恢复;注射后168 h,其恢复过程仍在进行。计数对照组和实验组不同免疫反应强度的LH细胞数和它们占LH细胞总数的百分数,并用统计学方法检验,发现其结果与上述一致。大剂量LHRH-A能刺激受孕大鼠垂体促性腺激素细胞释放大量LH,从而抑制卵巢合成孕酮的功能,并导致终止妊娠。本文支持这种看法,而且认为,这种对促性腺激素细胞分泌活动的影响是可以恢复的。

THE IMMUNOHISTOCHEMICAL CHANGE OF PITUITARY GONADOTROPIC CELLS IN THE PREGNANT RATS AFTER INJECTION OF A HIGH DOSAGE OF LUTEINIZING HORMONE RELEASING HORMONE ANALOGUE (LHRH-A)

The specific anti-rat LH, anti-rat FSH and anti-hCG sera were used in the peroxidase antiperoxidase (PAP) immunohistochemical method to observe the change of pituitary gonadotropic cells in pregnant rats after being injected by a high dosage of LHRH-A. It was observed that 24 hrs after injection the immunohistochemical reaction of gonadotropic cells weakened strikingly; 48 hrs after injection the immunohistochemical reaction began to recover; and 168 hrs after injection, the recovery was still in progress. The number of LH cells of different immunoreactive intensity and their percentage in total LH cells of the control and experimental groups were calculated and tested by statistical method. It was found that the results were the same as the above. Our observations support the view that the high dosage of LHRH-A can stimulate gonadotropic cells in rat to release a great amount of LH rapidly. It inhibits the synthesis of progesterone in the ovary of pregnant rat and results in termination of pregnancy. Moreover it is believed that the effect of high dosage of LHRH-A on the secretory function of gonadotropic cells can recover themseleves.