CCL21/CCR7轴促进人肺癌A549细胞的趋化与侵袭

目的：研究CCL21/CCR7轴对肺癌A549细胞定向趋化与侵袭活性的影响.方法：RT-PCR法从临床肺腺癌标本中扩增出CCR7编码区序列,定向克隆至载体pEGFP-N1中,稳定转染A549细胞,Boyden小室法检测转染前后A549细胞对CCL21的趋化和侵袭活性的改变.结果：CCL21作用下多聚碳酸酯膜背面的转染后A549细胞数明显多于转染前的A549细胞数.结论：CCL21/CCR7轴能够促进A549细胞的趋化与侵袭,其可能参与了肺癌淋巴结转移的过程.进一步研究CCL21/CCR7在肺癌中的作用将有助于阐明肺癌淋巴结转移的机制.

CCL21/CCR7 axis promotes chemotaxis and invasion of human lung adenocarcinoma A549 cells

Objective: To investigate the effects of CCL21/CCR7 axis on chemotaxis and invasion of human lung adenocarcinoma A549 cells. Methods: CCR7 coding domain was amplified from a patient with lung adenocarcinoma using RT-PCR, directionally cloned into pEGFP-N1 plasmid, and was stably transfected into A549 cells. Effects of CCL21/CCR7 axis on chemotaxis and invasion of A549 cells were investigated using Boyden chamber assay before and after transfection. Results: Under the influence of CCL21, the number of the transfected A549 cells permeating the septum of the Boyden chamber was significantly larger than that of A549 cells without transfection(P<0.01). Conclusion: CCL21/CCR7 axis could promote the chemotaxis and invasion of A549 cells, and it maybe play a role in lymph node metastasis of lung cancer. Further study of CCL21/CCR7 axis may help to elucidate the mechanism of lymph node metastasis in human lung cancer.