黑面神化学成分的研究

目的:研究中草药黑面神的化学成分.方法:黑面神嫩枝和叶子用95%乙醇回流提取,提取物分别用氯仿、正丁醇萃取,将氯仿部位和正丁醇部位分别进行硅胶柱层析,其中正丁醇部位的化合物采用重结晶、Sephadex LH-20柱层析方法进行纯化;通过IR、UV、MS、1HNMR和13CNMR等波谱解析方法以及和化合物标准品对比的方法进行结构鉴定.结果:从黑面神氯仿部位分离得到了8个化合物,分别鉴定为正丁基-β-D-吡喃果糖苷(n-butyl-β-D-fructopyranoside,Ⅰ)、乙基-β-D-吡喃果糖苷(ethyl-β-D-fructopyranoside,Ⅱ)、阿魏酸二十四烷醇酯(tetracosylferulate,Ⅲ)、β-谷甾醇(β-sitosterol,Ⅳ)、正三十二烷醇(n-dotriacontanol,V)、胡萝卜苷(daucosterol,Ⅵ)、熊果苷(arbutin,Ⅶ)和(-)-表儿茶素((-)-epicatechin,Ⅷ).结论:8个化合物均为首次从该植物中分离得到.

Study on Chemical Constituents from Breynia fruticosa

AIM:To investigate the chemical constituents from the aerial parts of Breyniafruticosa.. METHOD: Various chromatographic techniques were employed for isolation and purification of the constituents. The structures were elucidated by chemical evidence and spectral methods. RESULT: Eight compounds were obtained and identified by spectroscopic methods and compared with authentic samples as n-butyl-β-D-fructopyranoside, ethyl-β-D-fructopyranoside, tetracosylferulate, β-sitosterol, n-dotriacontanol, daucosterol, arbutin and (-)-epicatechin. CONCLUSlON:All compounds were firstly isolated from Breynia fruticosa (L.) Hook. f.