| 豚鼠颞骨三维重建的冰冻连续切片技术 |
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| 引用本文： | 黄玮,董明敏,刘宏建. 豚鼠颞骨三维重建的冰冻连续切片技术[J]. 南方医科大学学报, 2002, 22(9): 835-835,838 |
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| 作者姓名： | 黄玮 董明敏 刘宏建 |
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| 作者单位： | 1. 河南职工医学院,河南,郑州,450003
2. 郑州大学第一附属医院耳鼻咽喉科,河南,郑州,450052 |
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| 摘 要： | 本文介绍了一种改进的豚鼠颞骨冰冻连续切片技术。将颞骨标本用甲酸脱钙,在标本两侧放置定位线,然后将定位线与标本一起用明胶包埋。此技术具有切片薄、制片周期短、定位准确等优点,所得图像既具备切片的细节性,又能反映颞骨的空间特性,对颞骨解剖研究具有重要意义。
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| 关 键 词： | 医学影像 计算机三维重建 解剖 颞骨 冰冻切片 豚鼠 |
| 文章编号： | 1000-2588(2002)09-0835-01 |
| 修稿时间： | 2002-08-05 |
Improved three-dimension reconstruction of serial frozen tissue sections of the temporal bone of guinea pigs |
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| HUANG Wei,DONG Ming-min,LIU Hong-jianHenan Occupational Training Medical College,Zhengzhou ,China. Improved three-dimension reconstruction of serial frozen tissue sections of the temporal bone of guinea pigs[J]. Journal of Southern Medical University, 2002, 22(9): 835-835,838 |
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| Authors: | HUANG Wei DONG Ming-min LIU Hong-jianHenan Occupational Training Medical College Zhengzhou China |
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| Affiliation: | HUANG Wei,DONG Ming-min,LIU Hong-jianHenan Occupational Training Medical College,Zhengzhou 450003,China, Department of Otorhinolaryngology,First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052,China |
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| Abstract: | Objective To improve the serial frozen tissue sections of the temporal bone of guinea pigs for better three-dimension reconstruction of the computer images. Methods The temporal bone of the guinea pigs was first decalcified by formate before embedding with lines marking the position of the specimen in glutin at low temperature. Result and Conclusion Thinner slices, shorter period and more accurate orientation were achieved to highlight the spacial features of the temporal bone, thus facilitating the anatomical study of the temporal bone. |
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| Keywords: | medical image three-dimensional reconstruction computer-assisted anatomy temporal bone frozen tissue slice guinea pigs |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
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