| 日本血吸虫中国大陆株重组脂肪酸结合蛋白(sj-FABPc)的特性和抗原表位的分析 |
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| 引用本文： | 赵巍,关海玮,苏川,胡雪梅,沈蕾,陈淑贞,张兆松. 日本血吸虫中国大陆株重组脂肪酸结合蛋白(sj-FABPc)的特性和抗原表位的分析[J]. 中国人兽共患病杂志, 2000, 16(6): 6-8 |
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| 作者姓名： | 赵巍 关海玮 苏川 胡雪梅 沈蕾 陈淑贞 张兆松 |
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| 作者单位： | [1]宁夏医学院生物学教研室,银川 [2]南京医科大学江苏省医学分子生物学重点实验室,银川 |
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| 基金项目： | 本研究课题获江苏省重点实验室开放基金资助作者单位： |
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| 摘 要： | 目的 了解重组的日本血吸虫中国大陆株脂肪酸结合蛋白（ｒＳｊ－ＦＡＢＰｃ）的特性及预测其抗原表位。方法 以ＰＣＲ方法从Ｓｊ－ｃＤＮＡ文库中扩增Ｓｊ－ＦＡＢＰｃ基因片段，并亚克隆于载体ｐＧＥＭ－Ｔ，对其核苷酸序列进行测定。以ＧＯＬＤＫＥＹ软件和Ｓｗｉｓｓ－Ｍｏｄｅｌ数据库分析Ｓｊ－ＦＡＢＰｃ的核苷酸序列和其编码的蛋白质特性，预测重组抗原的表位。结果 核酸序列分析证明，克隆基因确为Ｓｊ－ＦＡＢＰｃ，并由
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| 关 键 词： | 日本血吸虫 序列分析 抗原表位 Sj－FABPc |
| 文章编号： | 1002-2694(2000)06-0006-03 |
STUDIES ON THE CHARACTERISTIC AND ANTIGENIC EPITOPE OF A ECOMBINANT PROTEIN OF FATTY ACID-BINDING PROTEIN (FABPc)FROM SCHISTOSOMA JAPONICUM |
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| ZHAO Wei,WU Haiwei,SU Chuan,et al. STUDIES ON THE CHARACTERISTIC AND ANTIGENIC EPITOPE OF A ECOMBINANT PROTEIN OF FATTY ACID-BINDING PROTEIN (FABPc)FROM SCHISTOSOMA JAPONICUM[J]. Chinese Journal of Zoonoses, 2000, 16(6): 6-8 |
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| Authors: | ZHAO Wei WU Haiwei SU Chuan et al |
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| Abstract: | Aim To analyse the characteristic and antigen epitope of recombinant antigen Sj-FABPc,Methods The cloned gene of FABP was amplified by PCR from a Schistosoma japonicum adult worm cDNA library and subcloned into plasmid pGEM-T.The sequence was analysed by GOLDKEY DNA and Protein Analytical Program and Swiss-Model Protein Modelling server in Internet.Results The Tesult indicated that the cloned gene was Sj-FABPc.Three possible antigenic epitopes and three sites of binding fatty acid,of recombinant antigen ofSj-FABPc,were predicted by computer.Conclusions The antigen epitopes of Sj-FABPc and the main sites of binding fatty acid may share same peptide. |
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| Keywords: | Schistosoma japonicum Fatty acid binding protein(FABP) Recombinant antigen Sequence analysis antigenic epitope |
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