肺动脉高压模型大鼠肺动脉平滑肌细胞内钙的改变

目的分析肺动脉高压时肺动脉平滑肌细胞Ryanod-ine受体Ca2+]i释放功能的改变。方法腹腔注射野百合碱建立大鼠肺动脉高压模型,原代培养肺动脉平滑肌细胞,Fura-2/AM负载培养细胞,荧光测钙技术测量Ryanodine受体激动剂对Ca2+]i变化的影响。结果10nmol.L-1Ry-anodine使对照组Ca2+]i平均增加(93.31±12.41)nmol.L-1,使PAH组Ca2+]i平均增加(141.71±13.59)nmol.L-1。两组样本Ca2+]i增加的数值差异有显著性(P<0.01);10mmol.L-1Caffine使对照组Ca2+]i平均增加(149.02±13.02)nmol.L-1,使PAH大鼠PASMC的Ca2+]i平均增加(191.2±21.26)nmol.L-1,两组样本Ca2+]i数值的变化差异有显著性(P<0.01)。结论肺动脉高压大鼠原代培养的肺动脉平滑肌细胞对Ryanodine受体激动剂的敏感性增强,提示肺动脉高压时Ryanodine受体释放Ca2+]i的功能发生了异常改变。

Alteration of [Ca2+]i in pulmonary artery smooth muscle of pulmonary artery hypertension rats

Aim To investigate the changes of Ca2+]i in the pulmonary artery smooth muscle cells of PAH rats induced by MCT.Methods PAH rat model was established by MCT intraperitoneal injection.The PASMCs were primarily cultured and loaded with Fura-2/AM.Effects of Ryanodine receptor agonists on intra-cellular calcium were measured by Fluorescence microscopy Results After being given 10 nmol·L-1 Ryanodine,the concentration of Ca2+]i in control group increased by(93.31±12.41)nmol·L-1;and the concentration in PAH group increased by(141.71±13.59)nmol·L-1(P<0.01);10 mmol·L-1Caffine could increase Ca2+]i in control group by(149.02±13.02)nmol·L-1,and increase Ca2+]i in PAH group by(191.2±21.26)nmol·L-1(P<0.01).Conclusion The results demonstrate that the sensitivity of PASMCs of PAH rats to RyR agonist increase markedly,and suggest that the RyR Ca2+ release probably is abnormal in pulmonary artery smooth muscle cells of PAH rats.