肺动脉高压模型大鼠肺动脉平滑肌细胞内钙的改变 |
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引用本文: | 承伟,李智.肺动脉高压模型大鼠肺动脉平滑肌细胞内钙的改变[J].中国药理学通报,2010,26(5). |
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作者姓名: | 承伟 李智 |
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作者单位: | 1. 辽宁医学院药学院,辽宁,锦州,121000 2. 中国医科大学药学院天然药物研究室,辽宁,沈阳,110001 |
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基金项目: | 国家自然科学基金课题 |
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摘 要: | 目的分析肺动脉高压时肺动脉平滑肌细胞Ryanod-ine受体Ca2+]i释放功能的改变。方法腹腔注射野百合碱建立大鼠肺动脉高压模型,原代培养肺动脉平滑肌细胞,Fura-2/AM负载培养细胞,荧光测钙技术测量Ryanodine受体激动剂对Ca2+]i变化的影响。结果10nmol.L-1Ry-anodine使对照组Ca2+]i平均增加(93.31±12.41)nmol.L-1,使PAH组Ca2+]i平均增加(141.71±13.59)nmol.L-1。两组样本Ca2+]i增加的数值差异有显著性(P<0.01);10mmol.L-1Caffine使对照组Ca2+]i平均增加(149.02±13.02)nmol.L-1,使PAH大鼠PASMC的Ca2+]i平均增加(191.2±21.26)nmol.L-1,两组样本Ca2+]i数值的变化差异有显著性(P<0.01)。结论肺动脉高压大鼠原代培养的肺动脉平滑肌细胞对Ryanodine受体激动剂的敏感性增强,提示肺动脉高压时Ryanodine受体释放Ca2+]i的功能发生了异常改变。
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关 键 词: | 肺动脉高压 Ryanodine受体 肺动脉平滑肌细胞 细胞内钙 激动剂 荧光测钙技术 |
Alteration of [Ca2+]i in pulmonary artery smooth muscle of pulmonary artery hypertension rats |
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Abstract: | Aim To investigate the changes of Ca2+]i in the pulmonary artery smooth muscle cells of PAH rats induced by MCT.Methods PAH rat model was established by MCT intraperitoneal injection.The PASMCs were primarily cultured and loaded with Fura-2/AM.Effects of Ryanodine receptor agonists on intra-cellular calcium were measured by Fluorescence microscopy Results After being given 10 nmol·L-1 Ryanodine,the concentration of Ca2+]i in control group increased by(93.31±12.41)nmol·L-1;and the concentration in PAH group increased by(141.71±13.59)nmol·L-1(P<0.01);10 mmol·L-1Caffine could increase Ca2+]i in control group by(149.02±13.02)nmol·L-1,and increase Ca2+]i in PAH group by(191.2±21.26)nmol·L-1(P<0.01).Conclusion The results demonstrate that the sensitivity of PASMCs of PAH rats to RyR agonist increase markedly,and suggest that the RyR Ca2+ release probably is abnormal in pulmonary artery smooth muscle cells of PAH rats. |
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Keywords: | pulmonary artery hypertension ryanodine receptor pulmonary artery smooth muscle cell intracellular calcium agonist fluorescence calcium measurement technique |
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