异氟烷预处理对心肺转流术后早期肺损伤的影响

Objective To observe the effects of isoflurane preconditioning against cardiopulmonary bypass (CBP)-induced early injury of lungs.Methods Sixteen patients undergone cardiac valve replacement were randomly divided into control group (group C,n =8) or isoflurane group (group I,n =8).Midazolam,fentanyl and vecuronium were used to maintain anesthesia.Isoflurane was inhaled for at least 30 min before CBP and then eluted out in group I,while only pure oxygen was inhaled in group C.Serum TNF-α,P-selectin,MDA and myeloperoxidase (MPO) were measured and respiratory index (RI,PA-aDO2/PaO2) was calculated according to blood gas analysis 10 min after anesthesia induction (T1),30 min (T2) and 180 min (T3) after vena cava opened,respectively.Before chest closure a bit of lung tissue was taken for detection of gap junction protein 43 (Cx43) and pathological examination.Results Serum TNF-α,P-selectin,MDA,MPO and RI significantly increased after CPB (T2-3) in both groups (P＜0.05-0.01),with less increasing extent in group I than in group C.There were no differences in distribution,colorful gap junction of lungs and CX43 expression in both groups.Pathological examination showed obvious edema in alveolar space,destroyed alveolar structure,severe neutrophil accumulation and erythrocyte infiltration in both groups.Conclusion Isoflurane preconditioning attenuates the oxidative stress response and lung inflammatory reaction,exerting protection of lungs against CBP-induced early injury.

Effects of isoflurane preconditioning against cardiopulmonary bypass-induced early injury of lungs

Objective To observe the effects of isoflurane preconditioning against cardiopulmonary bypass (CBP)-induced early injury of lungs.Methods Sixteen patients undergone cardiac valve replacement were randomly divided into control group (group C,n =8) or isoflurane group (group I,n =8).Midazolam,fentanyl and vecuronium were used to maintain anesthesia.Isoflurane was inhaled for at least 30 min before CBP and then eluted out in group I,while only pure oxygen was inhaled in group C.Serum TNF-α,P-selectin,MDA and myeloperoxidase (MPO) were measured and respiratory index (RI,PA-aDO2/PaO2) was calculated according to blood gas analysis 10 min after anesthesia induction (T1),30 min (T2) and 180 min (T3) after vena cava opened,respectively.Before chest closure a bit of lung tissue was taken for detection of gap junction protein 43 (Cx43) and pathological examination.Results Serum TNF-α,P-selectin,MDA,MPO and RI significantly increased after CPB (T2-3) in both groups (P＜0.05-0.01),with less increasing extent in group I than in group C.There were no differences in distribution,colorful gap junction of lungs and CX43 expression in both groups.Pathological examination showed obvious edema in alveolar space,destroyed alveolar structure,severe neutrophil accumulation and erythrocyte infiltration in both groups.Conclusion Isoflurane preconditioning attenuates the oxidative stress response and lung inflammatory reaction,exerting protection of lungs against CBP-induced early injury.