Immunomodulatory activity of andrographolide on macrophage activation and specific antibody response |
| |
Authors: | Wei WANG Jing WANG Sheng-fu DONG Chun-hong LIU Paola ITALIANI Shu-hui SUN Jing XU Diana BORAS-CHI Shi-ping MA Di QU |
| |
Affiliation: | [1]Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210038, China; [2]Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Institute of Medical Microbiology and Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University, Shanghai 200032, China; [3]Laboratory of Cytokines, Unit of Immunobiology, Institute of Biomedical Technologies, National Research Council, Pisa 56124, Italy; [4]Beijing Institute of Biological Products, Beijing 100024, China |
| |
Abstract: | Aim:To investigate the immunomodulatory effects of andrographolide on both innate and adaptive immune responses.Methods:Andrographolide (10 μg/mL in vitro or 1 mg/kg in vivo) was used to modulate LPS-induced classical activated (M1) or IL-4-induced alternative activated (M2) macrophages in vitro and humor immune response to HBsAg in vivo. Cytokine gene expression profile (M1 vs M2) was measured by real-time PCR, IL-12/IL-10 level was detected by ELISA, and surface antigen expression was evaluated by flow cytometry, whereas phosphorylation level of ERK 1/2 and AKT was determined by Western blot. The level of anti-HBs antibodies in HBsAg immunized mice was detected by ELISA, and the number of HBsAg specific IL-4-producing splenocyte was enumerated by ELISPOT.Results:Andrographolide treatment in vitro attenuated either LPS or IL-4 induced macrophage activation, inhibited both M1 and M2 cytokines expression and decreased IL-12/IL-10 ratio (the ratio of M1/M2 polarization). Andrographolide down-regulated the expression of mannose receptor (CD206) in IL-4 induced macrophages and major histocompability complex/costimulatory molecules (MHC I, CD40, CD80, CD86) in LPS-induced macrophages. Correspondingly, anti-HBs antibody production and the number of IL-4-producing splenocytes were reduced by in vivo administration of andrographolide. Reduced phosphorylation levels of ERK1/2 and AKT were observed in macrophages treated with andrographolide.Conclusion:Andrographolide can modulate the innate and adaptive immune responses by regulating macrophage phenotypic polarization and Ag-specific antibody production. MAPK and PI3K signaling pathways may participate in the mechanisms of andrographolide regulating macrophage activation and polarization. |
| |
Keywords: | macrophages cytokines antibodies immunomodulator andrographolide |
本文献已被 维普 等数据库收录! |
|