鼻咽癌组织与外周血CD4+CD25+调节性T细胞检测及临床意义

 目的 通过检测鼻咽癌患者肿瘤组织及外周血中CD4⁺T、CD8⁺T、CD4⁺CD25^-T、CD4⁺CD25⁺T细胞的频数,寻找客观、全面评价鼻咽癌患者免疫状态的临床指标。方法 采用流式细胞术检测40例初诊鼻咽癌患者及10例正常对照鼻咽部组织和外周血CD4⁺T、CD8⁺T、CD4⁺CD25^-T、CD4⁺CD25⁺T细胞比例。结果 鼻咽癌患者CD4⁺T细胞比例及CD4⁺/ CD8⁺T比值均低于对照组（P<0.05）,而CD8⁺T细胞两组间差异无统计学意义（P>0.05）,但是CD4⁺/ CD8⁺T比值在鼻咽癌组织与外周血间差异无统计学意义（P>0.05）。鼻咽癌组织及外周血中CD4⁺CD25⁺T细胞比例都高于对照组（P<0.05）,同时癌组织中该细胞比例远远高于外周血（P<0.05）。在鼻咽癌组织中CD4⁺CD25⁺T细胞与CD8+ T细胞、CD4⁺CD25^-T细胞呈负相关（r分别为-0.70、-0.675,P<0.05）,而在外周血中没有相关关系（P>0.05）。在不同T(原发肿瘤大小)组间,T4组的鼻咽癌组织中CD4⁺CD25⁺T细胞分别高于T1、T2、T3各组（P<0.05）,而在T1、T2、T3各组间差异无统计学意义（P>0.05）;鼻咽癌中CD4⁺CD25⁺T细胞比例与患者有无淋巴结转移并无关系（P>0.05）;鼻咽癌组织中Ⅲ+Ⅳ期组CD4⁺CD25⁺T细胞比例高于Ⅰ+Ⅱ期组（P<0.05）,而在外周血中两组间差异无统计学意义（P>0.05）。结论 CD4⁺CD25⁺T细胞与鼻咽癌病程进展无相关性,但是联合检测患者肿瘤组织及外周血中CD4⁺CD25⁺T细胞的频数并结合既往CD4⁺/ CD8⁺T比值会全面反应患者免疫状态,为临床治疗提供依据。

Detection and Clinical Significance of CD4+CD25+ Regulatory T Cells in Patients with Nasopharyngeal Carcinoma

Objective To detect the frequency of CD4⁺T,CD8⁺ T,CD4⁺CD25^-T,CD4⁺CD25⁺T cells in nasopharyngeal carcinoma (NPC), and to explore the objective clinical index to evaluate the immunity of NPC.Methods Flow Cytometry was to calculate the frequency of CD4⁺T,CD8⁺T,CD4⁺CD25-T,CD4⁺CD25⁺T cells in tumor tissue and peripheral blood from forty NPC and ten control.Results The proportion of CD4⁺T cells and ratio of CD4⁺/CD8⁺in NPC are significantly lower than those in control group（P<0.05）,but there was no difference in CD8⁺T cells between two groups（P>0.05）,there was also no significant difference in CD4⁺/CD8⁺between tumor tissue and peripheral blood in NPC（P>0.05）. The ratios of CD4⁺CD25⁺T cells in tissue and peripheral blood of NPC was higher than control group, and the ratio in tumor tissue was dramatically higher than in peripheral blood in NPC（P<0.05）.There was negative correlation between CD4⁺CD25⁺T cells and CD8⁺,CD4⁺CD25-T cells in tumor tissue of NPC respectively（r=-0.70,-0.675,P<0.05）,but this was not seen in peripheral blood （P>0.05）.According to the tumor sizes, patients with NPC were divided into four groups. The ratio of CD4⁺CD25⁺T cells in T4 tissue was higher than that in T1,T2,T3 respectively（P<0.05）,but there was no difference among T1,T2,T3 groups（P>0.05）.There was no correlation between frequency of CD4⁺CD25⁺T cells and lymphonode metastasis（P>0.05）.The ratio of CD4⁺CD25⁺T cells in tumor tissue at stage Ⅲ+Ⅳ was higher than the stage Ⅰ+Ⅱ of NPC（P<0.05）,but there was no difference between the two stage in peripheral blood in NP（P>0.05). Conclusion CD4⁺CD25⁺T cells was not correlated to the NPC prognosis, but detection of CD4⁺CD25⁺T cells in tissue and peripheral blood in NPC joint the ratio of CD4⁺/CD8⁺ which was used in the past will all round evaluate the immunity of NPC, It may provide evidence for clinical therapy.