Analysis by fluorescence in situ hybridization with a mouse gamma satellite DNA probe of isolated micronuclei induced in mice by two clastogens and two spindle poisons |
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| Authors: | Maki-Paakkanen Jorma; Hayashi Makoto; Suzuki Takayoshi; Tanabe Hideyuki; Honma Masamitsu; Sofuni Toshio |
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| Institution: | 1Division of Environmental Health, National Public Health Institute PO Box 95, FIN-70701 Kuopio, Finland
2Division of Genetics and Mutagenesis, National Institute of Health Sciences 1–18–1 Kamiyoga, Setagaya-ku, Tokyo 158, Japan |
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| Abstract: | The presence of centromeric DNA was studied in micronuclei isolatedfrom the blood of male ddY mic after five weekly intraperitonealinjections of mitomycu C (MMC), 1-ß-D-arabinofuranosylcytosine(Ara-C), colchi cine (COL) or vinblastine sulfate (VBL). Inagreement with our earlier findings, about half of the micronucleiisolate* from vehicle control mice showed centromere signalsa analyzed by fluorescence in situ hybridization (FISH) witha mouse major (gamma) satellite DNA probe. In an earlie experimentwith mice acutely exposed to the same chem icals, the clastogensMMC and Ara-C did not reduce thi proportion of micronuclei withcentromere signals. In the present study, however, MMC and Ara-Cdecreased the proportion of micronuclei with centromeres. Incontrast the spindle poisons COL and VBL increased the proportionof micronuclei that contained centromeres.
3To whom correspondence should be addressed |
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