Evaluation of Enzyme Immunoassays and Real-Time PCR for Detecting Shiga Toxin-Producing Escherichia coli in Southern Alberta,Canada |
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| Authors: | Linda Chui Laura Patterson-Fortin Julie Kuo Vincent Li Valerie Boras |
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| Affiliation: | aProvincial Laboratory for Public Health, Edmonton, Alberta, Canada;bUniversity of Alberta, Edmonton, Alberta, Canada;cDepartment of Laboratory Medicine, Chinook Regional Hospital, Lethbridge, Alberta, Canada |
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| Abstract: | Two immunoassays (Shiga Toxin Chek and Shiga Toxin Quik Chek) and real-time PCR were used to detect Shiga toxin-producing Escherichia coli. For enriched culture, the sensitivity and specificity of the three methods ranged from 80.0% to 98.2% and 98.0% to 100.0%, respectively. STEC isolates were identified in 2.6% of the 784 samples. |
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