Key differences in B cell activation patterns and immune correlates among treated HIV-infected patients versus healthy controls following influenza vaccination |
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| Institution: | 1. Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 29425, USA;2. Chief of No. 5 Biologicals Department, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kuming 650118, China;3. Division of Infectious Diseases, Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA;4. Hollings Cancer Center, Medical University of South Carolina, Charleston, SC 29425, USA;5. Division of Infectious Diseases, Department of Medicine, Case Western Reserve University, Cleveland, OH 41006, USA;6. Department of Drug Discovery and Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425, USA;7. Beijing You’an Hospital, Capital Medical University, No. 8 Xitoutiao, You’an men wai, Fengtai District, Beijing 100069, China;1. Department of Medical Rehabilitation, Pomeranian Medical University, Szczecin, Poland;2. Clinic of Neurology, Pomeranian Medical University, Szczecin, Poland;3. Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland;4. Clinic of Otolaryngology, Pomeranian Medical University, Szczecin, Poland;1. Laboratory of Building Environment and New Energy Resources, School of Civil Engineering, Dalian University of Technology, Dalian, Liaoning 116024, China;2. Tri-Y Environmental Research Institute, R & D Centre, 228-1820 Renfrew St., Vancouver, B.C., Canada V5M 3H9;3. ZhongXin United Automation (Dalian) Co., Ltd., Dalian, Liaoning 116024, China;1. Department of Oncology, Johns Hopkins Bayview Medical Center, Baltimore, MD 21224, USA;2. Medical Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA;3. Mouse Imaging Facility, National Institute of Neurological Disorders and Stroke, National Institutes of Health (NIH), Bethesda, MD 20892, USA;4. Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA;5. Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA;6. Division of Hematology-Oncology, Department of Medicine, Vanderbilt-Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37232, USA;1. Department of General Surgery, The Second Affiliated Hospital of Xuzhou Medical University, 32 Meijian Road, Xuzhou, 221006, Jiangsu, China;2. Department of General Surgery, XuZhou Central Hospital, 199 Jiefang Road, Xuzhou, 221009, Jiangsu, China;1. Laboratory of Malaria Immunology and Vaccinology, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD, United States;2. Laboratory of Clinical Infectious Diseases—Epidemiology Unit, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD, United States;3. MOMS Project, Seattle Biomedical Research Institute (currently Center for Infectious Disease Research), Seattle, WA, United States;4. Muheza Designated District Hospital, Muheza, Tanzania |
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| Abstract: | BackgroundThere is increasing recognition of the role of B cell dysfunction in HIV pathogenesis, but little is known about how these perturbations may influence responses to vaccinations.MethodsHealthy controls (n = 16) and antiretroviral therapy (ART)-treated aviremic HIV-infected subjects (n = 26) receiving standard-of-care annual influenza vaccinations were enrolled in the present study. Total bacterial 16 S rDNA levels were assessed by quantitative polymerase chain reactions in plasma. Serologic responses were characterized by ELISA, hemagglutination inhibition assay (HI), and microneutralization, and cell-mediated responses were assessed by ELISPOT (antigen-specific IgG+ antibody-secreting cells (ASCs)) and flow cytometry at pre-vaccination (D0), day 7–10 (D7) and day 14–21 (D14) post-vaccination.ResultsDecreased peripheral CD4+ T cell absolute counts and increased frequencies of cycling and apoptotic B cells were found at baseline in HIV-infected subjects relative to healthy controls. In healthy controls, post-vaccination neutralizing activities were related to the frequencies of vaccine-mediated apoptosis and cycling of B cells, but not to CD4+ T cell counts. In patients, both baseline and post-vaccination neutralizing activities were directly correlated with plasma level of bacterial 16S rDNA. However, overall vaccine responses including antibody titers and fold changes were comparable or greater in HIV-infected subjects relative to healthy controls.ConclusionB cell function correlates with measures of recall humoral immunity in response to seasonal influenza vaccination in healthy controls but not in ART-treated patients. |
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| Keywords: | HIV disease B cells Antibody responses Influenza vaccine |
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