An optimized,highly efficient,self-assembled,subvirus-like particle of infectious bursal disease virus (IBDV) |
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| Affiliation: | 1. Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China;2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, PR China;1. Vaccine Research Center, Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;2. National Center for Protein Science • Shanghai, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201210, China;1. Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, PR China;2. OIE Reference Laboratory for Infectious Bursal Disease, Avian and Rabbit Virology, Immunology and Parasitology Unit (VIPAC), Anses-French Agency for Food, Environmental and Occupational Health and Safety, Ploufragan, France;3. Experimental Poultry and Rabbit Unit (SELEAC), Anses-French Agency for Food, Environmental and Occupational Health and Safety, Ploufragan, France;1. Laboratory of Molecular Virology and Immunology, College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071000, China;2. Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071000, China;3. Department of Biotechnology, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China;4. Department of Dermatology, PLA Army General Hospital General Hospital, Beijing 100700, China;5. Rinpu (Baoding) Biological Pharmaceutical Co., LTD, Baoding 071004, China |
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| Abstract: | Infectious bursal disease virus (IBDV) causes immunosuppression in young chickens, leading to increased susceptibility to other diseases and a reduction in the immune response to other vaccines. Thus, IBDV results in great economic losses to the poultry industry. The most effective method of prevention is vaccination. However, medium-virulence vaccines can cause bursal pathological damage and immunosuppression. Here, we describe a safer, self-assembled, subvirus-like particle (sVP) vaccine without a complex purification process. The IBD-VP2 gene was cloned into Pichia pastoris, and the expressed protein self-assembled into T = 1 sVPs (∼23 nm). Immunization experiments showed that the sVP vaccine elicited high IBDV-neutralizing antibodies in each group, and all birds survived challenge with very virulent IBDV (vvIBDV). Additionally, IBDV RNA was not detected, and sterile immunity was achieved. In conclusion, the IBD-sVP is a suitable candidate for a recombinant subunit vaccine against IBDV. |
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| Keywords: | Infectious bursal disease virus Yeast sVP |
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