一氧化氮对全胃肠外营养肝脂肪变性的保护作用

目的探讨一氧化氮(NitricOxide,NO)对全胃肠外营养(TotalParenteralNutrition,TPN)引起的肝脂肪变性的作用。方法30只Wistar大鼠随机分为5组A组，正常对照；B组，TPN；C组，TPN+精氨酸；D组，TPN+N

Protection of nitric oxide for hepatic steatosis caused by total parenteral nutrition in rats

Objectives　To investigate the effect of nitric oxide(NO) on hepatic steatosis caused by total parenteral nutrition(TPN) in rats.Methods　Thirty Wistar rats were randomly divided into five groups: Group A,normal control; Group B,TPN group;Group C,TPN plus L-arginine; Group D,TPN plus NG-nitrio-L-arginine methyl ester(L-NAME，NOS inhibitor); Group E,TPN plus L-arginine and L-NAME.At the seventh day,liver function tests were performed and the livers were resected to test the lipid and nitric oxide content,and nitric oxide synthase activity.Results　The hepatic lipid content 〔triglycerid(μmol/g),cholesterol(μmol/g)〕in group B significantly increased compared with group A （39.3±2.4、13.1±1.1 vs.6.9±0.8、5.6±0.6）(P＜0.05).That was higher in group D（49.7±6.2、14.1±1.7）than in group B(P＜0.05),whereas that was lower in group C（18.2±4.4、7.3±3.1）than in group B(P＜0.05).The activity and distribution of NOS in the liver were associated with the content and distribution of hepatic lipid.Conclusions　NO produced by the liver may protect hepatic steatosis caused by total parenteral nutrition in rats.