低温胁迫下淡色库蚊体内蛋白表达的双向电泳分析

目的探索低温胁迫对淡色库蚊体内蛋白质表达的影响。方法以室内饲养淡色库蚊为对照,利用双向电泳技术,比较低温处理后淡色库蚊体内蛋白质表达谱的差异。结果处理组和对照组蛋白质浓度分别为8.5μg/μL和4.76μg/μL,二者差异有统计学意义(P<0.05)。经双向电泳分析,共发现2倍差异蛋白点77个,低温处理组表达量上调的蛋白点30个,表达量下调的蛋白点47个;低温处理组特有蛋白点14个,分子量主要在25~60 kDa之间。共鉴定出25个差异表达蛋白质,主要与细胞骨架的重塑、糖代谢、能量代谢以及各种合成酶和代谢酶有关。结论低温处理可诱导淡色库蚊体内蛋白质的表达变化,对差异蛋白的进一步分析有望获得淡色库蚊的抗冻蛋白。

Analysis of low temperature stress induced proteomic changes in Culex pipiens pallens via two-dimensional electrophoresis

Objective To explore the effects of low temperature stress on protein expression in Culex pipiens pallens.Methods Two-dimensional electrophoresis was performed to compare the differences in protein expression profiles of Cx.pipiens pallens after low temperature treatment using indoor bred Cx.pipiens pallens as control.Results The protein concentration was 8.5μg/μL and 4.76μg/uL respectively for the treatment group and the control group,and the difference was significant(P<0.05).Two-dimensional electrophoresis analysis revealed a total of 772-fold differential protein spots,in which up-regulated expression was seen in 30 protein spots,and down-regulation was observed in another 47 protein spots in low temperature treatment group.Fourteen unique protein spots were detected in the low temperature treatment group,with molecular weight ranging from 25 to 60 kDa.A total of 25 differentially expressed proteins were identified,mainly related to cytoskeleton remodeling,carbohydrate metabolism,energy metabolism,and various synthases and metabolic enzymes.Conclusion Low temperature treatment can induce changes in protein expression in Cx.pipiens pallens.Further analysis of the differential proteins may be promising to obtain antifreeze proteins from Cx.pipiens pallens.