拮抗血管内皮生长因子对骨折愈合影响的实验研究

为探讨拮抗血管内皮生长因子（VEGF）对骨折愈合的影响，制作兔左桡骨中段骨折模型，给实验组动物在3周内应用VEGF多克隆抗体（400ng腹腔注射，每3天1次），分别于伤后1、3、5、8周摄骨折部位X线片，并取骨折端标本脱钙进行光镜及电镜观察。结果显示，实验组动物伤后初期骨细胞坏死增多，虽然伤后72h骨折端成纤维细胞出现增生现象，但1－5周骨折端均有灶性坏死出现，3周时骨折部位血管生成明显减少，电镜下发现骨折端成纤维细胞和成骨细胞细胞器存在变性，伤后第8周骨折端以纤维组织连接为主。X线片上见骨不连样改变。提示骨折端VEGF表达障碍可能是导致骨不连的重要原因之一。

THE EFFECTS OF ANTI-VEGF ON FRACTURE HEALING

In order to explore the effect of anti vegf on fracture healing process,rabbits with left radius fracture were used as an animal model and divided randomly into control and experiment groups.VEGF polyclonal antibody was given to the animals of the experiment group films were thken at the fracture sites after 1,3,5 and 8 weeks of the fracture.the results showed that the cellular necrosis increased in the experiment group shortly after the fracture.Although there was fibroblast proliferation at 72 hours after the fracture,continuous focal necrosis was seen from 1 to 5 weeks in the fracture sites,and the vascularization reduced obviously 3 weeks after the injury.The organelle degeneration was observed in the fibroblasts and osteoblasts under microscope.The fracture sites were filled with fibrous tissue at the 8 th week and nonunion was ween in the fracture sites on X ray films.These results suggest that the lack of VEGF in the fracture sites might be one of the important reasons of fracture healing.