高通量分子捕捉系统研究中药的思路与方法

目的：本文提出以高通量分子捕捉系统研究中药的思路和方法。方法：以简单的分子系统和复杂的生物系统对信号分子捕捉和响应的特点为基础，分析了简单的单一蛋白质分子系统对中药混合物中的活性分子具有的特异性捕捉作用，蛋白质分子捕获活性分子后产生的信号变化可被检测系统检知，且反应易于分析，因此，以蛋白质分子为基本构件构建分子捕捉系统，可将目前所有药物作用机制涉及的约500种蛋白靶标分子构建成适于分析、研究的高通量分子捕捉阵列，从而对中药进行全方位研究。结果：将含药材料的获得和处理技术、高通量分子捕捉阵列、高通量检测技术和自动化数据处理系统整合、集成为高通量分子捕捉系统，可以突破现有研究方法的限制，对中药进行多方位的研究。结论采用高通量分子捕捉系统研究中药可突破目前中药研究的方法学限制但将此设想用于研究实践尚需大量工作：

Ideas and Methods in Study of Traditional Chinese Medicine by Capturing System of High Through -put Molecules

Objective: To explore ideas and methods in the study of traditional Chinese medicine by the capturing system of high through - put molecules. Method: To analyze the specially capturing functions of a simple system of unitary protein molecules on the active molecules in the compound of Chinese medicines on the basis of the characteristics which the simple molecular system and complex bio - system possess in their capture of and response to signal molecule. The change of signals emerging when protein molecules capture active molecules can be shown by the detecting system and be analyzed easily. Therefore, the molecule - capturing system made up by protein molecules as its basic components is able to form around 500 types of protein - targeting molecules involved in the mechanisms of all drugs into capturing arrays of high through - put molecules, which are suitable to analyses and studies. As a result Chinese herbal medicines can afford to be studied in an all - round way. Result: The processing technology, the capturing arrays of high through - put molecules, high through - put detecting technology and the automatically data - processing systems used in Chinese medicinal materials can be integrated into a high through - put molecule - capturing system which can break the barriers existing in the present methodology of studies so as to promote the study of traditional Chinese medicine in many ways. Conclusion: The study of Chinese medicines through high through - put molecule - capturing system is able to break restrictions in methodology applied in the current studies of Chinese drugs. It is necessary, however, to do plenty of work before such a system is put into practice.