| 骨髓间充质干细胞在博莱霉素致小鼠肺损伤的疗效观察 |
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| 引用本文： | 康秀华,张伟,曾省都.骨髓间充质干细胞在博莱霉素致小鼠肺损伤的疗效观察[J].江西医学院学报,2009,49(5). |
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| 作者姓名： | 康秀华 张伟 曾省都 |
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| 作者单位： | [1]南昌大学研究生院医学部 [2]南昌大学第一附属医院呼吸内科 [3]赣南医学院附属医院呼吸内科 |
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| 摘 要： | 目的观察骨髓间充质干细胞(BMSCs)对博来霉素致小鼠肺损伤动物模型的疗效。方法体外分离、培养雄性6周C57BL/6J小鼠间充质干细胞。15只雌性8周C57BL/6J小鼠随机分为3组:阴性对照组(P组),阳性对照组(B组),BMSCs治疗组(B+M组,每组5只)。P组于气管内注入PBS 50μL,B组及B+M组按5μg/g剂量气管内注入博莱霉素50μL制备肺纤维化模型,B+M组于造模后6 h经尾静脉注射BMSCs 1×107/mL,200μL。实验第28天统一处死小鼠,留取肺组织行病理HE染色、羟脯氨酸(hydroxyproline,HYP)含量测定,提取肺组织的DNA,通过聚合酶链反应(PCR)-琼脂糖电泳法检测雄鼠的性别决定基因(sry基因),以判断外源给予的BMSCs是否在雌性小鼠肺组织中存在。结果①肺组织病理:B+M组的肺泡炎及肺纤维化程度均较B组减轻。②HYP含量:B组的HYP含量明显高于P组及B+M组,B+M组的HYP含量与P组无明显差异。③B+M组有雄性小鼠的sry基因存在。结论①外源性BMSCs经尾静脉注射可以到达肺损伤部位;②外源性BMSCs可以减轻肺泡炎、肺纤维化的程度。
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| 关 键 词： | 特发性肺纤维化 骨髓间充质干细胞 羟脯氨酸 sry性别决定区 动物 实验 小鼠 |
Effect of Mesenchymal Stem Cells on Bleomycin-induced Lung Injury in Mice |
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| Institution: | KANG Xiu-hua1a; ZHANG Wei1b; ZENG Xing-du2(1a.2006 Grade of Medical Department of Graduate School; 1b.Department of Respiratory; the First Affiliated Hospital; Nanchang University; Nanchang 330006; China; 2.Department of Respiratory; the Affiliated Hospital; Gannan Medical College; Ganzhou 341000; China); |
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| Abstract: | Objective To explore the effects of bone marrow-derived mesenchymal stem cells(BMSCs) on bleomycin-induced pulmonary fibrosis in mice.Methods BMSCs were isolated from 6-week-old male C57BL/6J mice and cultured in culture flasks.The fourth passage mesenchymal stem cells(MSCs) were used in this experiment.15 C57BL/6J mice were randomly divided into 3 groups: negative control group(Group P),positive control group(Group B),MSCs transplantation group(Group B+M),with 5 mice in each group.The mice in Group P were ... |
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| Keywords: | idiopathic pulmonary fibrosis bone-marrow derived mesenchymal stem cells hydroxyproline sex-determining region in Y chromatosome animals laboratory mice |
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