| 大鼠脑缺血血管内皮生长因子与NG2细胞增殖关系的初步研究 |
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| 引用本文： | 焦岩,胡俊,范沁林,刘国军,黄河清,陈康宁. 大鼠脑缺血血管内皮生长因子与NG2细胞增殖关系的初步研究[J]. 第三军医大学学报, 2010, 32(2) |
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| 作者姓名： | 焦岩 胡俊 范沁林 刘国军 黄河清 陈康宁 |
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| 作者单位： | 第三军医大学西南医院神经内科,重庆,400038 |
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| 摘 要： | 目的观察大鼠脑缺血后少突胶质前体细胞(oligodendrocyte progenitor cells,OPC)的表达,并初步探讨血管内皮生长因子(vascular epithelial growth factor,VEGF)在其中的作用。方法实验动物72只,按随机数字表法分为假手术对照组、缺血组和缺血+贝伐单抗组共3组,每组设6、12、24、72 h 4个时相点,每个时相点6只大鼠,采用激光共聚焦观察缺血后不同时相点NG2和VEGF免疫荧光标记的细胞增殖状况。结果在大脑皮层区,NG2与VEGF标记细胞基本完全重叠。在缺血后72 h内,阳性标记细胞随时间延长,进行性增多,其中贝伐单抗组在各时相点的双阳性细胞数均明显少于缺血组(P<0.05)。结论脑缺血损伤后脑皮层区存在OPC的活化现象。VEGF可能对损伤后NG2的增殖有促进作用。
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| 关 键 词： | 脑缺血 少突胶质前体细胞 血管内皮生长因子 |
Role of VEGF in NG2 cells proliferation in rats after cerebral ischemia |
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| Jiao Yan,Hu Jun,Fan Qinlin,Liu Guojun,Huang Heqing,Chen Kangning. Role of VEGF in NG2 cells proliferation in rats after cerebral ischemia[J]. Acta Academiae Medicinae Militaris Tertiae, 2010, 32(2) |
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| Authors: | Jiao Yan Hu Jun Fan Qinlin Liu Guojun Huang Heqing Chen Kangning |
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| Affiliation: | Department of Neurology;Southwest Hospital;Third Military Medical University;Chongqing;400038;China |
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| Abstract: | Objective To observe the reactive changes of oligodendrocyte progenitor cells(OPCs) in rats after cerebral ischemia,and explore the effects of vascular epithelial growth factor(VEGF) in these cells.Methods Totally 72 rats were randomly divided into sham-operation control(SC) group,ischemia group and ischemia+bevacizumab group(2 mg/kg,intraperitoneally injected after the operation).The ischemia model was established by bilaterally ligating common cervical artery.The 6 animals of each group were sacrificed re... |
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| Keywords: | brain ischemia oligodendrocyte progenitor cells vascular epithelial growth factor |
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