补肾益智方对老年性痴呆细胞模型的影响

背景 在体外培养的神经细胞培养液中加入具有神经毒性的淀粉样蛋白(amyliod protein,Aβ)片段可诱导产生阿尔茨海默病 (Alzheimer's disease,AD)相似的病理损害,补肾药是否改善其病理损害是本研究探讨的问题.目的观察补肾益智方对由 Aβ片段毒性诱导的 NG10815细胞老年性痴呆模型作用的影响.设计完全随机对照实验研究. 地点和材料研究地点为广西中医学院第二附属医院神经科学研究所,材料为健康三月龄Wista大鼠 60只,体质量 200～ 250 g,购自第一军医大学动物实验室.干预与主要观察指标 将 Wista大鼠随机分为对照组、治疗组,每组 30只,雄雌各半.利用细胞计数、MTT及流式细胞仪测定等方法观察补肾益智方含药血清处理由 Aβ片段毒性诱导的NG108-15细胞的存活率、形学改变及细胞周期的改变. 结果 补肾益智方含药血清能增加Aβ片段毒性诱导的 NG108-15细胞存活率和细胞突起;细胞增殖周期分析显示,含药血清能增加S期细胞数. 结论 补肾益智方具有减轻细胞对 Aβ的神经毒性反应,表明该方在通过拮抗AD的病理发展而发挥对 AD的治疗作用.

Effect of bushen yizhi fang,a traditional Chinese medicinal formula,on a cell model of Alzheimer disease

BACKGROUND: In vitro cultured nerve cells induced by neurotoxic amyliod β protein(Aβ) fragments exhibit pathological changes similar to those seen in Alzheimer disease(AD) . This study examines the effect of kidney-tonifying traditional Chinese medicine in alleviating the pathological lesions in AD.OBJECTIVE: To assess the effect of bushen yizhifang, a traditional Chinese medicinal formula, on the growth behavior and morphology of neurotoxic Aβ-treated NG108-15 cells as an AD model.DESIGN: Completely randomized and controlled experimental study.SETTING and MATERILAS: The experiment was conducted in the Institute of Neuroscience, Second Affiliated Hospital of Guangxi College of Traditional Chinese Medicine. Sixty Wistar rats, 3 months old and weighing 200 g to 250 g, were purchased from the Experimental Animal Center of First Military Medical University of Chinese PLA.INTERVENTIONS and MAIN OUTCOME MEASURES: The rats were randomized into.control and treatment group, with 30 rats in each and totally gender-balanced. The rats in the latter group were given gastric infusion of the extract of bushen yizhi fang for a month before their blood was collected for preparing the drug-containing serum. Changes of Aβ-induced NG108-15cells, such as the viability rate, morphology and cell cycle, were assessed by cell counting, MTT assay and flow cytometry, respectively, after treatment of the cells with the prepared rat serum.RESULTS: The viability rate and number of cell processes of Aβ-induced NG108-15 cells were increased after treatment with drug-containing serum. Cell cycle analysis indicated an increased in S-phase cell number after the treatment.CONCLUSION: Bushen yizhi fang may alleviate the neurotoxicity of Aβ-segments in the nerve cells, and therefore produces therapeutic effect on AD by inhibiting the pathological progression.