大鼠异位心脏移植（Ono术式）的流程及经验体会

目的总结建立大鼠同种异位心脏移植模型的经验、方法及体会。方法行大鼠同种异位心脏移植（Ono术式）120例。其中预备实验60例，正式实验60例。结果预备实验手术成功率为45％，正式实验手术成功率为85％。正式实验整个手术时间为（74．3±17．1）min，供心缺血时间（29．6±t0．3）min，供心摘取时间约（7．9±4．2）min。移植心存活时间为（11．1±5．7）d；5例心脏移植后大鼠长期存活，最大体重达740g。结论成功制备大鼠心脏移植模型，良好手术技术和严格的围手术期处理同等重要。

Experience and procedure of the model of rat heterotopic heart transplantation

Objective To summarize the method and experience of the model of rat heterotopic heart transplantation and to improve the rate of success of the experiment. Methods 120 heterotopic heart transplantations were performed by 0no＇s way, including 60 prepared transplantations and 60 formal traMs-plantations. Results Rate of success of prepared transplantations was 45%, and formal transplantation was 85%. Mean time of formal operation was （74. 3 ± 17. 1 ）min, removal time of the donor heart was （7. 9 ± 4. 2 ） rain, ischemia time was （ 29. 6± 10. 3 ） min and the survival time of allograft was （ 11.1 ± 5.7 ） days. 5 of the recipients had a long-term survival, and the maximum weight was 740 g. Conclusions Good sur- gical technique and rigorous perioperative management is very important in setting up the rat model of heter- otopic heart transplantation.