| CEA真核表达载体的瞬时表达及检测 |
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| 引用本文： | 袁云,宋晓妍,张娓,闫志勇,张钦宪.CEA真核表达载体的瞬时表达及检测[J].河南医学研究,2005,14(4):306-308. |
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| 作者姓名： | 袁云 宋晓妍 张娓 闫志勇 张钦宪 |
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| 作者单位： | 郑州大学基础医学院组织学与胚胎学教研室,河南,郑州,450052;郑州大学基础医学院组织学与胚胎学教研室,河南,郑州,450052;郑州大学基础医学院组织学与胚胎学教研室,河南,郑州,450052;郑州大学基础医学院组织学与胚胎学教研室,河南,郑州,450052;郑州大学基础医学院组织学与胚胎学教研室,河南,郑州,450052 |
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| 摘 要： | 目的:检测含癌胚抗原(CEA)的真核表达质粒pWACEA在体外的瞬时表达。方法:用脂质体包裹含CEA的真核表达质粒pWACEA转染MCF7细胞,采用原位杂交和免疫组化技术检测CEA的表达。结果:转染细胞原位杂交和免疫组化均为阳性;对照标本未见阳性信号。结论:真核表达质粒pWACEA在体外可表达CEA分子,为进一步建立CEA阳性动物肿瘤细胞模型及研究CEA阳性肿瘤的免疫防治提供了基础。
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| 关 键 词： | 癌胚抗原 质粒表达 原位杂交 |
| 文章编号： | 1004-437X（2005）04-0306-03 |
| 收稿时间： | 2005-09-27 |
| 修稿时间： | 2005-11-01 |
Transient expression of eukaryotic expression plasmid CEA in vitro and its detection |
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| YUAN Yun,SONG Xiao-yan,ZHANG Wei,YAN Zhi-yong,ZHANG Qin-xian.Transient expression of eukaryotic expression plasmid CEA in vitro and its detection[J].Henan Medical Research,2005,14(4):306-308. |
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| Authors: | YUAN Yun SONG Xiao-yan ZHANG Wei YAN Zhi-yong ZHANG Qin-xian |
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| Institution: | Department of Histology and Embryology, Basic Medical College of Zhengzhou University , Zhengzhou 450052, China |
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| Abstract: | Objective: To detect expression of the eukaryotic expression plasmid pWA-CEA in vitro. Methods: MCF-7 cell line was transfected by this mammalian expression plasmid pWA-CEA using lipofectamine reagent. The expression of CEA molecule was detected by in situ hybridization and immunocytochemistry technique. Results: The positive signals were found in MCF-7 cells, but no positive signals were exhibited in contrast specimens.Conclusion: CEA molecule can be expressed in vitro after transfection,which lay a good foundation for constructing animal CEA positive tumor cell model and studying the prevention and therapy of CEA positive tumor. |
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| Keywords: | eareinoembryonie antigen plasmid expression in situ hybridization |
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