| 聚乙二醇修饰牛胰核糖核酸酶 |
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| 引用本文： | 李伟军,张颖,王云山,何明磊,苏志国.聚乙二醇修饰牛胰核糖核酸酶[J].药物生物技术,2001,8(3):147-151. |
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| 作者姓名： | 李伟军 张颖 王云山 何明磊 苏志国 |
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| 作者单位： | 中国科学院化工冶金研究所，生化工程国家重点实验室，北京 100080 |
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| 基金项目： | 国家自然科学基金（批准号：29736180） |
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| 摘 要： | 采用N-羟基珀酰亚胺活化酯法活化单甲氧基聚乙二醇，测定了聚乙二醇（PEG）的活化度为86.2%。以活化的PEG对牛胰核糖核酸酶进行化学修饰；分析了蛋白质被修饰程度。用毛细管电泳法给出了被修饰蛋白的修饰度与修饰蛋白分布的定量结果。比较了被修饰产物对大分子底物(酵母RNA)与小分子底物（2',3'-环磷酸胞嘧啶）的降解活力，其表观酶活力分别保留了52.8%和66.3%。结合毛细管电泳定量分析得到的修正酶活力略低于表观酶活力。
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| 关 键 词： | 聚乙二醇 牛胰核糖核酸酶 化学修饰 毛细管电泳 酶活性 |
| 文章编号： | 1005-8915(2001)03-0147-05 |
| 修稿时间： | 2001年3月8日 |
Modification of Ribonuclease A by Methoxypolyethylene Glycol |
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| LI Wei-jun,ZHANG Ying,WANG Yun-shan,HE Ming-lei,SU Zhi-guo.Modification of Ribonuclease A by Methoxypolyethylene Glycol[J].Pharmaceutical Biotechnology,2001,8(3):147-151. |
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| Authors: | LI Wei-jun ZHANG Ying WANG Yun-shan HE Ming-lei SU Zhi-guo |
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| Abstract: | Bovine pancreatic ribonuclease A (RNase) was modified with N-hydroxysucci n imide-activated methoxypolyethylene glycol (MPEG).The activation degree of MP EG was determined as 86.2%.The MPEG-RNase was analyzed by TNBS assay, SDS-PAG E an d capillary electrophoresis (CE).CE was chosen as efficient method for determi n ing the modification degree and characterizing the heterogeneity of MPEG-RNase . The apparent retained enzymatic activities of MPEG-RNase towards yeast RNA and c ytidine 2',3'-cyclic monophosphate were determined as 52.8% and 66.3%, respe cti vely.The revised enzymatic activities calculated by subtracting the proportion of residuary unmodified RNase according to CE analysis were also shown in this p aper. |
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| Keywords: | Polyethylene glycol Chemical modification Ribonuclease A Capillary electropho resis Enzymatic activity |
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