环境烟草烟雾暴露对小鼠脑区神经递质表达的影响

目的 通过观察小鼠脑部神经递质的变化,初步探讨环境烟草烟雾(ETS)成分对小鼠神经系统的影响.方法 在染毒实验台内对小鼠染毒,染毒8周造模后进行小鼠脑区定位,免疫组织学方法和图像分析观察脑区神经递质表达的变化结果 (1)GABA在小鼠大脑皮层、海马部位的表达:ETS暴露组阳性细胞数量明显减少(大脑皮层:0.25±0.10;海马:0.19±0.07)(P＜0.05).(2)nAChR在大脑皮层的表达:ETS吸入组阳性表达明显增加(0.31±0.10)(P＜0.05).(3)NMDAR在小鼠大脑皮层、纹状体皮质的表达:ETS吸入组(0.31±0.08,0.35±0.11)、尼古丁(Nic)吸入组阳性表达增加明显(P＜0.05).结论 ETS、Nic可导致大脑皮层、下丘脑等部位神经递质及受体表达的变化.

Abstract：

Objective To explore the mechanism of the influence on mice brain of environmental tobacco smoke. Methods After the mice were placed into the bench for 8 weeks, the region of the mice brain was localized and the expression of neurotransmitters and neurotransmitters receptors were detected by immunohistochernistry.Results ( 1 ) The expression of GABA in the mice cerebral cortex ( CC ) ( 0. 25 ± 0. 06 ) and the hippocampus (Hip) (0. 19 ± 0. 07 ) were much higher in the ETS-exposed group than that in the control group(P＜ 0. 05 ). (2)The expression of nAChR on CC(0. 31 ±0. 10) was much more in the ETS-exposed group than that in control group(P＜0.05). (3) The expression of NMDAR( Glu receptors) on the CC and striate cortex were much higher in the ETS-exposed group(0.32 ±0. 10,0.38 ±0. 14), NIC-inhaling group(0.31 ±0. 08,0.31 ± 0. 11 ) than that in control group(P＜0. 05 ). Conclusions Long-term ETS-exposed and NiC-exposed environment could change the expression of neurotransmitter and its receptors.

The influence of experimental tobacco smoke exposure on brain neurotransmitters in mice

Objective To explore the mechanism of the influence on mice brain of environmental tobacco smoke. Methods After the mice were placed into the bench for 8 weeks, the region of the mice brain was localized and the expression of neurotransmitters and neurotransmitters receptors were detected by immunohistochernistry.Results ( 1 ) The expression of GABA in the mice cerebral cortex ( CC ) ( 0. 25 ± 0. 06 ) and the hippocampus (Hip) (0. 19 ± 0. 07 ) were much higher in the ETS-exposed group than that in the control group(P＜ 0. 05 ). (2)The expression of nAChR on CC(0. 31 ±0. 10) was much more in the ETS-exposed group than that in control group(P＜0.05). (3) The expression of NMDAR( Glu receptors) on the CC and striate cortex were much higher in the ETS-exposed group(0.32 ±0. 10,0.38 ±0. 14), NIC-inhaling group(0.31 ±0. 08,0.31 ± 0. 11 ) than that in control group(P＜0. 05 ). Conclusions Long-term ETS-exposed and NiC-exposed environment could change the expression of neurotransmitter and its receptors.