补阳还五汤对局灶性脑缺血小鼠血管生成和Ang-1/Tie-2表达的影响

目的 观察补阳还五汤对局灶性脑缺血小鼠血管生成和血管生成素-1(Ang-1)及其受体Tie-2表达的影响.方法 35只KM小鼠分成假手术组(n=10),模型组(n=12)和补阳还五汤组(n=13).大脑中动脉阻塞(MCAO)30 min诱导小鼠局灶性脑缺血,缺血后24h开始灌服补阳还五汤,1次/d,缺血后28d内分别采用神经症状评分和角实验评价神经功能;缺血后第28天,采用免疫组化检测缺血周边区微血管密度和Ang-1/Tie-2的表达.结果 缺血后第3～28天,补阳还五汤能显著改善神经症状和减少小鼠右转次数,与模型组比较差异有显著性(P＜0.05);脑缺血后28d,模型组CD31、Ang-1、和Tie-2免疫阳性细胞增多分别为(437±59)个,(389±61)个,(251±42)个],而补阳还五汤组为分别为(609±68)个,(551±66)个,(342±46)个],两组之间差异有显著性(P＜0.01).结论 补阳还五汤促进脑缺血后神经功能恢复可能与其上调Ang-1/Tie-2表达促进缺血周边区血管生成有关.

Abstract：

Objective To investigate the effect of Buyanghuanwu decoction(BYHWD) on angiogenesis and Ang-1/Tie-2 expression after focal cerebral ischemia in mice. Methods Focal cerebral ischemia was induced by 30 min of middle cerebral artery occlusion followed by reperfusion. BYHWD (20 g/kg) was administered orally 24 h after ischemia and once a day. The neurological score and the corner test were used to evaluate sensorimotor function during 28 days after ischemia. The microvessels density and the expression of Ang-1/Tie-2 in the ischemic boundary zone were determined by immunohistochemistry on 28 days after ischemia. Results Compared with the model group, BYHWD significantly ameliorated neurological dysfunction and reduced the number of right turn during 3 to 28 days after ischemia(P＜0.05 ). In addition,the numbers of CD31 ,Ang-1 and Tie-2 immunopositive cells were (437 ±59) ,(389 ±61 ) and (251 ±42) at the boundary zone in model group 28 days after ischemia,respectively. However, BYHWD treatment significantly increased the numbers of CD31(609 ± 68 ), Ang-1 (551 ±66) and Tie-2 (342 ±46) immunopositive cells(P＜0. 01 ). Conclusion BYHWD promotes angiogenesis,which may contribute to recovery of neurological function after focal cerebral ischemia,and Ang-1/Tie-2 pathway appears to mediate BYHWD-induced angiogenesis.

Effect of buyanghuanwu decoction on angiogenesis and Ang-1/Tie-2 expression after focal cerebral ischemia in mice

Objective To investigate the effect of Buyanghuanwu decoction(BYHWD) on angiogenesis and Ang-1/Tie-2 expression after focal cerebral ischemia in mice. Methods Focal cerebral ischemia was induced by 30 min of middle cerebral artery occlusion followed by reperfusion. BYHWD (20 g/kg) was administered orally 24 h after ischemia and once a day. The neurological score and the corner test were used to evaluate sensorimotor function during 28 days after ischemia. The microvessels density and the expression of Ang-1/Tie-2 in the ischemic boundary zone were determined by immunohistochemistry on 28 days after ischemia. Results Compared with the model group, BYHWD significantly ameliorated neurological dysfunction and reduced the number of right turn during 3 to 28 days after ischemia(P＜0.05 ). In addition,the numbers of CD31 ,Ang-1 and Tie-2 immunopositive cells were (437 ±59) ,(389 ±61 ) and (251 ±42) at the boundary zone in model group 28 days after ischemia,respectively. However, BYHWD treatment significantly increased the numbers of CD31(609 ± 68 ), Ang-1 (551 ±66) and Tie-2 (342 ±46) immunopositive cells(P＜0. 01 ). Conclusion BYHWD promotes angiogenesis,which may contribute to recovery of neurological function after focal cerebral ischemia,and Ang-1/Tie-2 pathway appears to mediate BYHWD-induced angiogenesis.