EGF和bFGF诱导骨髓间充质干细胞向神经细胞分化后对Tau和神经元特异性烯醇化酶表达的影响

目的：观察表皮细胞生长因子（BGF）、碱性成纤维生长因子（bFGF）体外诱导骨髓间充质干细胞（MSCs）分化为神经细胞后，神经元微管相关蛋白Tau和神经元特异性烯醇化酶（NSE）的表达。方法：取P4代MSCs分为EGF、bFGF和EGF＋bFGF及对照组；免疫细胞化学法检测Tau蛋白和NSE表达，ELISA法分析各组细胞Tau蛋白含量。结果：免疫细胞化学检测显示EGF＋bFGF811Tau蛋白和神经元特异性烯醇化酶NSE阳性细胞率最高，bFGF组、EGF组和对照组依序递减；各组Tau蛋白含量结果与Tau蛋白阳性细胞率一致。结论：EGF和bFGF均可促进MSCs向神经细胞分化，并表达Tau蛋白和NSE，二者具有协同作用。

Expression of Tau protein and neuron-specific enolase after differentiation of mesenchymal stem cells into neural cells induced by EGF and bFGF

Objective: To observe the expression of Tau protein and neuron-specific enolase (NSE) after differentiation of bone-marrow mesenchymal stem cells (MSCs) into neural cells induced by EGF and bFGF in vitro. Methods: P4-MSCs were divided into 4 groups: EGF+bFGF, bFGF, EGF, and control groups. After 14-day induction, positive rates of Tau protein and NSE were tested by immunocytochemistry and Tau protein content assay was analyzed with ELISA test. Results : It was found that the positive rates of Tau protein and NSE in EGF+bFGF group were the highest, followed by bFGF, EGF and control groups in turn (P<0. 001). In eachgroup, the content of Tau protein was in consistent with its positive rate. (P<0. 001). Conclusion: EGF and bFGF can induce MSCs into neural cells in vitro and express Tau protein and NSE. They have synergistic effects during the inducing process.