| HPLC测定不同采收期荭草中荭草素、异荭草素的含量 |
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| 引用本文： | 窦妍,翟延君,佟苗苗,张慧.HPLC测定不同采收期荭草中荭草素、异荭草素的含量[J].中国实验方剂学杂志,2012,18(2):62-64. |
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| 作者姓名： | 窦妍 翟延君 佟苗苗 张慧 |
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| 作者单位： | 辽宁中医药大学药学院,辽宁大连,116600 |
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| 基金项目： | 国家自然科学基金项目(30873437) |
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| 摘 要： | 目的:建立高效液相色谱法同时测定不同采收期荭草中荭草素、异荭草素含量的方法.方法:色谱柱为AgilentC18(4.6 mm×150 mm,5μm),流动相乙腈-0.1％磷酸水梯度洗脱,检测波长350 nm,流速1.0 mL· min -1.结果:荭草素在0.068 2 ～0.682 μg(r=0.999 7)线性关系良好,平均回收率为99.94％,RSD 1.08％；异荭草素在0.386～3.860 μg(r=0.999 3)线性关系良好,平均回收率为99.67％,RSD 1.09％.结论:方法简便、快捷、准确的特点,可用于荭草药材的质量控制.
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| 关 键 词： | 荭草 荭草素 异荭草素 含量测定 高效液相色谱法 |
| 收稿时间： | 2011/7/31 0:00:00 |
Determination of Orientin and Isoorientin in Different Harvest Times of Polygonum orientale by HPLC |
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| DOU Yan,ZHAI Yan-jun,TONG Miao-miao and ZHANG Hui.Determination of Orientin and Isoorientin in Different Harvest Times of Polygonum orientale by HPLC[J].China Journal of Experimental Traditional Medical Formulae,2012,18(2):62-64. |
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| Authors: | DOU Yan ZHAI Yan-jun TONG Miao-miao and ZHANG Hui |
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| Institution: | Liaoning University of Traditional Chinese Medicine, Dalian 116600, China;Liaoning University of Traditional Chinese Medicine, Dalian 116600, China;Liaoning University of Traditional Chinese Medicine, Dalian 116600, China;Liaoning University of Traditional Chinese Medicine, Dalian 116600, China |
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| Abstract: | Objective:To establish an HPLC method for the simultaneous determination of orientin and isocrientin in different harvest times of Polygonum orientale.Method:Orientin and isoorientin were separated on Agilent C18(4.6 mm×150 mm,5μm) column and detected at 350 nm.The mobile phase was acetonitrile-0.3% phosphoric with gradient elution.The flow rate was 1.0 mL·min-1.Result: Orientin and isoorientin were linear within the range of 0.068 2-0.682 μg(r=0.999 7),0.386-3.860 μg(r=0.999 3)respectively.The average recovery was 99.94%(1.08%),99.67%(1.09%).Conclusion: The method is simple,rapid,and it can be used for the quality control of P.orientale. |
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| Keywords: | Polygonum orientale L orientin isoorientin determination HPLC |
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