水蒸气蒸馏与超临界CO2萃取香胶木叶 挥发油化学成分的GC-MS分析

目的:考察水蒸气蒸馏法(SD)和超临界萃取法(SFE-CO2)得到的香胶木叶挥发油化学成分差别.方法:用GC-MS对香胶木叶挥发油进行测定,并用色谱峰面积归一化法测定各成分的相对百分含量.结果:SD获得挥发油中分离出33个峰,鉴定了其中19个组分,占提取物总量的71.94％,其中的主要成分是叶醇(43.90％),青叶醛(7.42％),2,4-二叔丁基苯酚(4.80％)等.SFE-CO2获得的挥发油中分离出55个峰,鉴定了其中33个组分,占提取物总量的81.51％,其中的主要成分是角鲨烯(11.85％),桉油精(9.95％),(+)-α-松油醇(5.34％)等.结论:两种提取方法得到的香胶木叶挥发油组分与相对含量差别较大,该实验结果为了解其挥发性成分及进一步开发应用提供了依据.

Chemical Constituents in Essential Oil from Litsea glutinosa by Steam Distillation and SFE-CO2 by GC-MS

Objective: To compare chemical constituents of the essential oil of Litsea glutinosa (Lour.) C.B.Rob. were extracted by steam distillation (SD) and supercritical-CO₂ fluid extraction (SFE-CO₂). Method: The chemical components of the essential oil were separated and identified by GC-MS, Their relative contents were determined by normalization of peak area. Result: In essential oil extracted by SD 33 peak were separated and 19 of them were identified representing 71.94% of the total contents. The volatile constituents were mainly the (Z)-3-Hexen-1-ol (43.90%), (E)-2-Hexenal (7.42%), 2,4-bis(1,1-dimethylethyl)-Phenol (4.80%). In essential oil extracted by SFE-CO₂ 55 peak were separated and 33 of them were identified representing 81.51% of the total contents. The volatile constituents were mainly the squalene (11.85%), eucalyptol (9.95%), (+)-.alpha.-Terpineol (5.34%). Conclusion: The constituents of essential oils extracted by SFE-CO₂ were different from that of extraction by SD. The result provided evidence for understanding the constituents of essential oils and explitation of Litsea glutinosa (Lour.) C.B.Rob.