基于炎症反应的香椿子总多酚抗大鼠 心肌缺血再灌注损伤的机制研究

目的:观察香椿子总多酚对大鼠心肌缺血再灌注急性炎症的影响.方法:50只SD大鼠随机分成假手术组(冠脉下穿线不结扎+0.5％羧甲基纤维素钠)、模型组(冠脉结扎+0.5％羧甲基纤维素钠)、香椿子总多酚低剂量(XD,50 mg· kg-1+冠脉结扎)、中剂量(XZ,100 mg·kg-1+冠脉结扎)、高剂量(XG,200 mg· kg-1+冠脉结扎)组,各组均ig给药.采用左冠状动脉前降支结扎30 min再灌120 min的方法复制大鼠心肌缺血再灌注损伤模型.以ST抬高作为结扎成功的标志,以ST段逐渐回落、梗死性Q波出现作为再灌注成功的标志.再灌结束后行腹主动脉取血,分离血清,测定血清中白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)含量.取左心室缺血区组织10 mg用RT-PCR测定核因子kB (NF-kB)p65 mRNA的表达水平,其余心肌组织做病理切片,并在光镜下观察左心室心肌组织的形态学变化.结果:模型组大鼠血清IL-6含量为(638.88±188.94) ng·L-1,XD,XZ,XG组与模型组相比,血清IL-6含量显著下降,分别为(491.58±142.59),(306.85±80.60),(246.11 ±71.64) ng·L-1.模型组大鼠血清TNF-α含量为(216.23 ±58.67) ng·L-1,XD,XZ,XG组大鼠血清TNF-α水平与之相比显著降低,分别为(148.24 -±36.30),(157.62 ±52.06),(144.10 ±36.64) ng·L-1.模型组大鼠心肌组织中NF-kBp65/β-actin为0.61 ±0.05,香椿子总多酚给药组大鼠NF-kB p65/β-actin与之相比显著降低,分别为0.53 ±0.08,0.45 ±0.13,0.38 ±0.11.香椿子总多酚组与模型组相比,大鼠心肌细胞的形态学损伤较轻.结论:香椿子总多酚能够减轻大鼠心肌缺血再灌注急性炎症,对其产生一定的保护作用.

Mechanism of Total Polyphenols Extracted from Toona sinensis Roem on Acute Inflammation during Myocardial Ischemia-reperfusion in Rats

Objective:To investigate effects of total polyphenols extracted from Toona sinensis Roem on acute inflammation in injury induced by myocardial ischemia-reperfusion in rats.Method: Fifty SD rats were randomly divided into 5 groups: sham operation group,model group and total polyphenols extracted from T.sinensis groups in 3 doses.Myocardial ischemia/reperfusion injury in rats was induced by legating the left coronary artery(LAD) 30 min followed by a 120 min reperfusion,while rats in sham group were sutured only.Ligature success was indicated by elevation of segment,yet reperfusion success was indicated through gradual resolution of T wave with ST segment,and appearance of infract Q wave.After reperfusion,blood from abdominal aorta was collected,and serum were prepared.Then content of IL-6 and TNF-α in serum were detected.Finally,10 mg myocardium tissue was cut from left ventricle ischemic area,and expression level of NF-κB p65 mRNA was tested.The rest myocardium tissues were prepared for pathological sections.Then the morphological changes in left ventricle ischemic area was observed through optical Microscope.Result:Content of IL-6 and TNF-α in model group serum were(638.88±188.94),(216.23±58.67) ng·L-1 respectively.Compared with rats in model group,after administrated with polyphenols extracted from T.sinensis Roem,the content of IL-6 and TNF-α in serum was declined.NF-κB p65/β-actin in left ventricle ischemic area myocardium tissue of model group was 0.61±0.05,while myocardium tissue NF-κB p65/β-actin in total polyphenols extracted from T.sinensis groups were decreased significantly.Compared with model group,the morphological injury of cardiomyocytes in total polyphenols extracted from T.sinensis groups were alleviated significantly.Conclusion: Total polyphenols extracted from T.sinensis can play an intervention role on acute inflammation during myocardial ischemia-reperfusion in rats.