| HPLC测定雪山金罗汉止痛涂膜剂中延胡索乙素的含量 |
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| 引用本文： | 倪琳,杨锡.HPLC测定雪山金罗汉止痛涂膜剂中延胡索乙素的含量[J].中国实验方剂学杂志,2012,18(2):106-108. |
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| 作者姓名： | 倪琳 杨锡 |
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| 作者单位： | 甘肃省食品药品检验所甘肃省中药品质与安全评价工程技术研究中心,兰州,730000 |
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| 摘 要： | 目的:建立雪山金罗汉止痛涂膜剂中延胡索乙素的含量测定方法.方法:采用HPLC法对制剂中主要成分延胡索乙素进行定量分析.CAPCELL PAK C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1％磷酸溶液(用三乙胺调节pH至6.0)(38∶62),检测波长280 nm,柱温30℃,流速1.0 mL· min -.结果:延胡索乙素在0.010 84～0.130 08μg呈良好的线性关系,平均回收率为97.74％,RSD 1.75％ (n =-9).结论:方法准确可靠,专属性强,结果稳定,重复性好,可有效控制制剂质量.
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| 关 键 词： | 雪山金罗汉止痛涂膜剂 延胡索乙素 高效液相色谱 |
| 收稿时间： | 2011/6/12 0:00:00 |
Determination of Tetrahydropalmatine in Xueshan Jinluohan Zhitong Balm by RP-HPLC |
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| NI Lin and YANG Xi.Determination of Tetrahydropalmatine in Xueshan Jinluohan Zhitong Balm by RP-HPLC[J].China Journal of Experimental Traditional Medical Formulae,2012,18(2):106-108. |
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| Authors: | NI Lin and YANG Xi |
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| Institution: | Gansu Provincial Institute of Food and Drug Control, Gansu Province Traditional Chinese Medicine Quality and Safety Evaluation Technology Research Center, Lanzhou 730000, China;Gansu Provincial Institute of Food and Drug Control, Gansu Province Traditional Chinese Medicine Quality and Safety Evaluation Technology Research Center, Lanzhou 730000, China |
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| Abstract: | Objective:To establish an method for determination of tetrahydropalmatine in Xueshan Jinluohan Zhitong Balm.Method: The tetrahydropalmatine in Xueshan Jinluohan Zhitong Balm was determined by HPLC.CAPCELL PAK C18(4.6 mm×250 mm 5 μm)column was used.The modbile phase was acetonitrile-0.1% phosphoric acid(adjusted pH to 6.0 with triethyplamine)(38∶62) with the flow rate of 1.0 mL·min-1.The column temperature was 30 ℃ and UV detection wavelength was at 280 nm.Result:The tetrahydropalmatine were linear at the range of 0.010 84-0.130 08 μg,respectively.The average recovery was 97.74% and RSD was 1.75%(n=9).Conclusion:The established method is accurate,reliable and specific.The quality of Xueshan Jinluohan Zhitong Balm can be controlled by the method. |
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| Keywords: | Xueshan Jinluohan Zhitong Balm tetrahydropalmatine HPLC |
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