疏风解毒颗粒防治手足口病的体内外药效学研究

目的:通过观察疏风解毒颗粒在体内对手足口病病毒EV71感染BALB/c乳鼠模型的保护作用和在体外对EV71等肠道病毒的抑制作用,评价疏风解毒颗粒防治手足口病的药效.方法:采用疏风解毒颗粒3岁儿童临床2倍、等倍和1/2倍剂量(14.08,7.04,3.52 g·kg-1)分别对手足口病病毒EV71H株感染的BALB/c乳鼠手足口病动物模型进行预防性给药(ig,3d)和治疗性给药(ig,5d),观察乳鼠感染的严重程度、死亡数,计算动物死亡率和生命延长率；采用疏风解毒颗粒在体外对Vero细胞无明显毒性的5个剂量,对人手足口病病毒EV71H株,柯萨奇病毒B3株,B4株,B5株的致Vero细胞病变的抑制作用进行观察.结果:疏风解毒颗粒对手足口病病毒EV71感染的乳鼠模型具有明显的预防和治疗作用,可降低感染程度和死亡率,具有明显的保护和延长生命的作用；疏风解毒颗粒在无明显毒性的浓度下,在体外对人手足口病病毒EV71 H株、CoxB3株、CoxB5株均具有明显的抑制作用.结论:疏风解毒颗粒在体内外均具有较好的防治手足口病的作用.

Pharmacodynamics Research on Preventive and Therapeutic Effects of Shufeng Jiedu Granules on Hand-Foot-Mouth Disease

Objective: To evaluate the preventive and therapeutic effects of Shufeng Jiedu granules on hand-foot-mouth disease by observing the death protective effect of the granules on suckling BALB/c mice infected by enterovirus 71 in vivo and the inhibition effect on enteric viruses in vitro. Method: Prophylactic and therapeutic administration of Shufeng Jiedu granules were applied to EV71 H strain infection suckling BALB/c mice at 2, 1 and 1/2 clinical equivalent dose of 3-year-old children respectively. We observed the degree of infection, death number and calculated the death rates and life extension rates. The in vitro inhibition of Shufeng Jiedu granules at five non-toxic dosages anti EV71 H strain, CoxB3 strain, CoxB4 strain and CoxB5 strain were determined by CPE method on Vero cells. Result: Shufeng Jiedu granules could decrease the degree of infection and death rates,simultaneously increase life extension rates significantly. Shufeng Jiedu granules showed significant preventive and therapeutic effects on suckling BALB/c mice infected by enterovirus 71.At no-toxic dosages, Shufeng Jiedu granules inhibited CoxB3 strain, CoxB4 strain and CoxB5 strain visibly. Conclusion: Shufeng Jiedu granules show notable preventive and therapeutic effects on hand-foot-mouth disease in vivo and in vitro.