鼠疫菌pYC质粒对菌体蛋白质组影响的初步研究

目的 了解鼠疫菌pYC质粒对菌体蛋白质组的作用。方法 将含有pYC质粒的鼠疫菌与不含该质粒的鼠疫菌通过双向电泳的方法进行蛋白质组比对，并将差异蛋白进行质谱鉴定。结果 含有pYC质粒的鼠疫菌与不含该质粒的鼠疫菌的蛋白图谱可识别的蛋白点均在500个以上，两者总体上相似性较高;前者较后者在大小约70×103、等电点5.0左右处，明显多一个蛋白点簇，该蛋白簇经质谱鉴定均为伴侣蛋白GroEL，但该蛋白并非是pYC质粒编码蛋白。结论 pYC质粒对菌体蛋白质组有影响，其编码蛋白pYC_p10与pYC_p11，可能调控GroEL高表达。

Preliminary study of the impact of plasmid pYC on proteome of Yersinia pestis

Objective To investigate the role of plasmid pYC on proteome of Yersinia pestis. Methods Two dimensional electrophoresis was performed to strains of Yersinia pestis with and without the pYC plasmid, and differential proteins were identified by mass spectrometry. Results More than 500 protein spots of Yersinia pestis with and without plasmid pYC were recognized, and their protein profiles were generally similar. The chaperone GroEL was highly expressed in strains with plasmid pYC, whereas the protein GroEL was not encoded by plasmid pYC. Conclusions Plasmid pYC has an impact on proteome of Yersiniapestis. The proteins of pYC-p10 and pYC-p11 encoded by plasmid pYC may regulate the expression of GroEL.