<Emphasis Type="Italic">Toxoplasma gondii</Emphasis>: expression and characterization of a recombinant protein containing SAG1 and GRA2 in <Emphasis Type="Italic">Pichia pastoris</Emphasis> |
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Authors: | Huaiyu Zhou Qinmin Gu Qunli Zhao Jiaqin Zhang Hua Cong Ying Li Shenyi He |
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Institution: | (1) Department of Parasitology, School of Medicine, Shandong University, Jinan, 250012, People’s Republic of China |
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Abstract: | Toxoplasma gondii is an obligate intracellular protozoan which infects most species of warm-blooded animals and causes toxoplasmosis. Previous
immunological and immunization studies have demonstrated the potential role of T. gondii antigens SAG1 and GRA2 as a vaccine candidate. In the present study, we have cloned, expressed, and purified a recombinant
protein SAG1–GRA2 in Pichia pastoris. Results showed that P. pastoris was a robust system producing a large amount of highly purified and biological activity protein. BALB/c mice immunized with
SAG1–GRA2 elicited stronger humoral and cellular responses in comparison to control groups. This immunization resulted in
an enhanced Th1 immune response as measured by IgG2a antibody production and increased splenocyte IFN-γ production, whereas
no IL-4 was detected. After a lethal challenge with the highly virulent T. gondii RH strain, a prolonged survival time in SAG1–GRA2-immunized mice was observed in comparison to control groups. Our data demonstrate
that SAG1–GRA2 triggered a protective response against toxoplasmosis. Therefore, SAG1–GRA2 protein might be a good candidate
for the further development of a multiantigenic vaccine. |
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