消疣颗粒对U_（14）宫颈癌小鼠免疫功能影响的实验研究

目的观察消疣颗粒对U14荷瘤小鼠外周血IL-2、TNF-α含量及对外周血T细胞亚群的影响。方法将模型小鼠随机分为消疣颗粒高、中、低剂量组,模型对照组,阳性对照组,连续给药11 d。用夹心ELISA法检测U14荷瘤小鼠外周血IL-2含量,双抗夹心ELISA法检测U14荷瘤小鼠外周血TNF-α含量,流式细胞仪检测U14荷瘤小鼠外周血CD4＋、CD8＋计数及CD4＋/CD8＋比值。结果消疣颗粒各处理组血清中TNF-α与IL-2的含量显著增高,消疣颗粒中、低剂量组CD4＋数量显著增加,CD8＋数量明显减少,CD4＋/CD8＋比值显著增加,对T淋巴细胞具有增殖的作用。结论消疣颗粒能调节荷瘤小鼠的免疫功能,达到治疗肿瘤的目的。

Experimental Research of the Impacts of Xiaoyou Granules on the Immune Function in the Mice with U14 Cervical Cancer

Objective To observe the impacts of xiaoyou granules on the contents of IL-2 and TNF-α in peripheral blood as well as the impacts on T-cell subgroups in U14 cervical cancer bearing mice.Methods The model mice were randomized into a high-dose xiaoyou granules group,a middle-dose xiaoyou granules group,a low-dose xiaoyou granules group,a model control group and a positive control group.The medication was continued for 11 days.The sandwich ELISA was adopted to detect the content of IL-2 in peripheral blood in U14 cervical cancer bearing mice.The double antibodies ELISA was used to detect the content of TNF-α in peripheral blood in U14 cervical cancer bearing mice.The flow cytometry(FCM)was applied to detect the counts of CD4+ and CD8+ in peripheral blood and the ratio of CD4+/CD8+ in U14 cervical cancer bearing mice.Results The contents of TNF-α and IL-2 in serum in every xiaoyou granules group were increased significantly.CD4+ count was increased significantly,CD8+ count was reduced apparently,the ratio of CD4+/CD8+ was increased significantly and the proliferation of T lymphocytes presented in the middle-dose and low-dose xiaoyou granules groups.Conclusion Xiaoyou granules regulate the immune function of cervical cancer bearing mice and achieve the goal of cancer treatment.