| 器官培养在研究人食管上皮癌变中的应用 |
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| 引用本文： | 裴许芳,周传农,王秀琴,吴旻.器官培养在研究人食管上皮癌变中的应用[J].中国医学科学院学报,1987(3). |
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| 作者姓名： | 裴许芳 周传农 王秀琴 吴旻 |
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| 作者单位： | 中国医学科学院肿瘤研究所 北京
(裴许芳,周传农,王秀琴),中国医学科学院肿瘤研究所 北京(吴旻) |
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| 摘 要： | 将组织块置于明胶海绵上,摇摆器官培养,4～6周后成人食管粘膜仍保持良好的结构形态,具有活跃生长的上皮细胞和良好的腺体结构;将组织块贴壁,静置外植块—细胞培养,成人食管粘膜和胎儿食管组织块可存活2～3个月,提供大量生长旺盛的上皮细胞。成人食管癌旁粘膜培养利于观察细胞癌前改变,可能较易于诱发癌变;胎儿食管具有正常背景,利于研究细胞癌变的全过程.二者配合可组成研究人食管上皮癌变的完整体外实验系统。
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| 关 键 词： | 食管上皮 器官培养 细胞培养 癌变 |
Application of Organ Culture in Stndying Human Esophagus Carcinogenesis |
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| Pei Xufang,Zhou Chuannong,Wang Xiuqin and Wu Min.Application of Organ Culture in Stndying Human Esophagus Carcinogenesis[J].Acta Academiae Medicinae Sinicae,1987(3). |
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| Authors: | Pei Xufang Zhou Chuannong Wang Xiuqin and Wu Min |
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| Abstract: | In this paper, two systems for culturing human esophagus tissues are described, One being organ culture of adult human esophageal "mucosa on surgical sponge and rocking; another being explant-cell culture of adult and fetal esophageal mucosa on dishes in a medium with low Ca++ and low serum contents. The results showed well growing basal epithelial cells of mucosa for 4-6 weeks with retaining of original structures in organ culture; while in explant-cell culture, mucosa could be maintained for 2-3 months and gave rise to luxuriant epithelial cells. The authors suggest to use the two systems in studies of precancerous lesions of esophageal epithelium on genetic background and malignant transformation in vitro. |
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| Keywords: | esophngenl mucosn organ culture epithelial cells culture carcinogensis model |
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