大肠癌组织中P16蛋白和血管内皮生长因子的表达及其临床意义

目的：探讨大肠癌组织中P16蛋白和血管内皮生长因子（VEGF）表达及其临床意认。方法：用S－P免疫组织化学方法测定66例大肠癌组织和20例正常大肠组织中P16蛋白和VEGF的表达。结果：大肠癌中P16蛋白阳性率为48．5％（32／66）明显低于对照组的70．0％（14／20）（P＜0．01），VEGF阳性率为72．7％（48／66）则明显高于对照组的15．0％（3／20）（P＜0．01）：P16蛋白和VEGF在大肠癌中表达具有明显负相关性；P16蛋白和VEGF表达与大肠癌组织学类型、肿瘤直径、肿瘤部位无关（P＞0．05），而与淋巴结转移、Duke's分期五年生存率有明显的关系（P＜0．01）。结论：大肠癌中存在P16蛋白下调和VEGF上调，P16蛋白和VEGF表达可作为反映大肠癌生物学行为的指标之一。

The expression of p16 protein and vascular endothelial growth factor in colorectal carcinoma tissue

Objective To investigate The expression of pl6 protein and vascular endothelial growthfactor (VEGF) in colorectal carcinoma tissues and its clinical significance Methods The expression of P16 protein and VEGF were detected in 66 cases of colorectal carcinoma and 20 cases of normal colorectal tissues by S-P immunohistochemistry. Results The positive rate of P16 protein (48.5%)in colorectal carcinoma tissues was significantly lower than that in the normal colorectal tissues(75.0%)(P<0.01), The positive rate of VEGF (72.7%)in corectal carcinoma tissues was significantly higher than that in the normal tissues(lS.0%)(P<0.01).There was a significant positive correlation between the expression of P16 protein and VEGF. No correlation was observed between the expression of them in histological type, tumor diameter and tumor place (P>0.05),but there was significant correlation with lymph node metastasis, clinical stage(Duke's) and the patients'five-year-survival probability(P<0.01). Conclusion There is a significant correlation between P16 protein expression down regulation and VEGF expression up regulation in colorectal carcinoma tissues. The expression of P16 protein and VEGF is one of the predictors for the biological behavior of colorectal carcinoma.