Substance P-, somatostatin- and calcitonin gene-related peptide-like immunoreactivity and fluoride resistant acid phosphatase-activity in relation to retrogradely labeled cutaneous, muscular and visceral primary sensory neurons in the rat |
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| Authors: | C Molander J Ygge C J Dalsgaard |
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| Institution: | 1. Department of Anatomy, Karolinska Institute, Stockholm Sweden;2. Department of Histology, Karolinska Institute, Stockholm Sweden;1. Department of Nutrigenomics and Bromatology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Jednosci 8, 41-200 Sosnowiec, Poland;2. Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Jednosci 8, 41-200 Sosnowiec, Poland;3. Institute of Electrical Drives and Machines KOMEL, 188 Rozdzienskiego Street, 40-203 Katowice, Poland;4. Department of Medical Genetics, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Jednosci 8, 41-200 Sosnowiec, Poland;1. Department of Pharmacology, Hebei Medical University, Shijiazhuang, 050011, PR China;2. Faculty of Medicine and Health, University of Leeds, Leeds, UK;3. School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, UK;1. School of Biotechnology, Southwest University, Chongqing 400716, China;2. School of Life Sciences, Southwest University, Chongqing 400716, China |
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| Abstract: | The distribution of several peptides in cutaneous, muscular and visceral primary sensory neurons was investigated in the adult rat. The fluorescent dye Fast blue was applied to the proximal ends of transected saphenous (cutaneous), gastrocnemius (muscular) and greater splanchnic (visceral) nerves. Sections from corresponding dorsal root ganglia were incubated for simultaneous indirect immunocytochemical demonstration of calcitonin gene-related peptide (CGRP)-, substance P (SP)- or somatostatin (SOM)-like immunoreactivity (-LI) and Fast blue. In addition, the presence of fluoride resistant acid phosphatase (FRAP)-enzyme activity (-EA) in retrogradely Fast blue-labeled saphenous and gastrocnemius nerves was investigated by subsequent enzyme cytochemical analysis. The results revealed the presence of CGRP-LI, SP-LI, SOM-LI and FRAP-EA in cell bodies of primary sensory neurons which project to the saphenous and gastrocnemius nerves. CGRP-LI and SP-LI, but not SOM-LI, were found in splanchnic sensory neurons. The vast majority of the visceral sensory neurons were found to contain CGRP-LI. |
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