| 用RAPD技术分析利什曼原虫DNA多态性 |
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| 引用本文： | 罗萍,胡孝素,章亚京,高蓉. 用RAPD技术分析利什曼原虫DNA多态性[J]. 寄生虫病与感染性疾病, 1998, 0(3) |
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| 作者姓名： | 罗萍 胡孝素 章亚京 高蓉 |
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| 作者单位： | 四川省卫生管理干部学院寄生虫学教研室，华西医科大学寄生虫学研究室 |
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| 基金项目： | 国家自然科学基金,四川省卫干院科研基金 |
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| 摘 要： | 为了建立ＲＡＰＤ技术分析利什曼原虫ＤＮＡ多态性的方法，并进一步揭示Ｌｉｎｆａｎｔｕｍ和Ｌｔｒｏｐｉｃａ之间的亲缘关系，本文应用筛选的两种随机引物（Ｍ１３，３３０１）分别扩增了Ｌｉｎｆａｎｔｕｍ和Ｌｔｒｏｐｉｃａ的ｎＤＮＡ和ｋＤＮＡ。结果显示，两种利什曼原虫ｎＤＮＡ及ｋＤＮＡ均扩增出各自特有的带型，两虫种间ｎＤＮＡ、ｋＤＮＡ的扩增片段共享度Ｆ值分别为０７２７２和０８５７１。表明ＲＡＰＤＰＣＲ可作为利什曼原虫虫种分析鉴别的工具
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| 关 键 词： | 利什曼原虫 nDNA及kDNA RAPDPCR |
ANALYSIS OF LEISHMANIA DNA BY RANDOM AMPLIFIED POLYMORPHIC DNA ASSAY |
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| Luo Ping ,Hu Xiaosu ,Zhang Yajing ,Gao Rong. ANALYSIS OF LEISHMANIA DNA BY RANDOM AMPLIFIED POLYMORPHIC DNA ASSAY[J]. Parastoses and Infectious Diseases, 1998, 0(3) |
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| Authors: | Luo Ping Hu Xiaosu Zhang Yajing Gao Rong |
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| Affiliation: | Luo Ping 1,Hu Xiaosu 2,Zhang Yajing 1,Gao Rong 1 1. Department of Parasitology,Sichuan Continuing Education College of Medical Sciences,Chengdu 610041 2. Department of Parasitology,West China University of Medical Sciences,Chengdu 610041 |
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| Abstract: | The random amplified polymorphic DNA (RAPD) assay has been used to find molecular markers and to detect L.infantum and L.tropica. Both nuclear DNA and kinetoplast DNA were analysed by RAPD using two arbitrary primers.The results showed that the two primers yielded characteristic patterns for nDNA and kDNA between L.infantum and L.tropica and there were some differences of the share random amplified DNA fragments, which demonstrated that RAPD PCR was a useful tool to analyse Leishmania DNA. |
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| Keywords: | Leishmania nDNA kDNA RAPD PCR |
| 本文献已被 CNKI 等数据库收录！ |
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