反向间接血凝法检测难辨梭菌B毒素

从高产毒难辨梭菌菌株的透析培养液中提纯了B毒素,并用之制备免抗血清,建立了反向间接血凝试验以检测该菌B毒素。该试验具有操作简便、省时及经济等优点;其特异性和敏感性与经典的组织培养法相比分别为88.9％和87.2％;对B毒素最低检出浓度为87ng/ml。可初步替代组织培养法并作为厌氧培养法的一项补充。

Reverse passive hemoagglutination for detection of clostridium difficile toxin B

A toxin-producing strain of clostridium difficile was anaerobically cultured in dialysis flasks. The culture supernatant was applied to filtrate chromatography and ion exchange chromatography sequentially. The toxin B was purified and was then used to immunize rabbits for antiserum. A reverse passive hemoagglutination (RPHA) was established, using the sheep red blood cells sensitized by the antibody. After 137 samples were tested, the RPHA assay showed the specificity and sensitivity of 88.9% and 87.2% respectively, as compared with tissue culture assay results. The detecting limit for purified toxin B was 78ng/ml. The test can be used as a screening test for a clinical lab.