Survivin在胰腺癌组织中的表达及其与p53的相关性研究

目的 :探讨凋亡抑制蛋白survivin在胰腺癌组织中的表达和p5 3表达的关系。方法 :用免疫组织化学方法Envision法对 5 1例胰腺导管癌survivin和p5 3的表达进行检测。结果 :胰腺导管癌中survivin蛋白的表达率为 80 .4 % ,p5 3的表达率为 6 0 .8% ;二者在 11例癌旁非肿瘤胰腺组织中均未发现阳性表达。survivin的表达与组织学分级有关 (P <0 .0 1) ,而与临床分期和淋巴结转移关系不大 (P >0 .0 5 ) ;P5 3的表达与淋巴结转移有关 (P <0 .0 5 ) ,而与组织学分级和临床分期关系不大 (P >0 .0 5 ) ;sur vivin的表达与p5 3的表达密切相关 (P <0 .0 1)。结论 :Survivin和p5 3突变对凋亡抑制的协同作用可能在胰腺癌的发生发展过程中起关键作用。

Survivin expression and its correlation with p53 in pancreatic adenocarcinoma

Objective: To investigate the expression of survivin and p53 in human pancreatic adenocarcinoma and its correlation with clinicobiological behavior. Methods: The expression of survivin and p53 in 51 cases of human pancreatic ductal adenocarcinoma were detected with immunohistochemistry of Envision . Results: Expression of survivin and p53 in pancreatic ductal adenocarcinoma were 80.4% and 60.8% respectively; no expression of survivin and p53 in adjacent normal tissue was detected. The survivin expression was significantly associated with histological grade (P<0.01)but not with clinical stages and lymph node metastasis status (P>0.05). The p53 expression was significantly correlated with lymph node metastasis status (P<0.05)but not with histological grades and clinical stages(P>0.05). The expression of survivin was closely correlated with p53 (P<0.01). Conclusion: The inhibition of apoptosis caused by cooperation between survivin and loss of p53 may play a pivotal role in tumorigenesis and tumor progression in pancreatic cancer.