人卵泡液诱导的顶体反应、精子形态与体外受精率的关系

目的:探讨人卵泡液诱导的顶体反应、精子形态及体外受精率3者之间的相互关系。方法:应用Spearm an法对79对不育患者的人卵泡液诱导的顶体反应、精子形态(采用结晶紫染色及精子形态严格分类标准)及体外受精率等指标进行等级相关分析。结果:人卵泡液诱导的顶体反应率与正常形态精子百分率之间存在极显著正相关(n=49,r=0.376 3,P<0.01),但人卵泡液诱导的顶体反应率与体外受精率以及正常形态精子百分率与体外受精率之间均未见显著相关性(n分别为21和50,r分别为0.266 6和0.001 8,P均>0.05)。结论:人卵泡液诱导的顶体反应率与正常形态精子百分率之间存在极显著正相关,但两者与体外受精率之间均未见显著相关性。

Relationships among Human Follicular Fluid-Induced Acrosome Reaction, Sperm Morphology and In Vitro Fertilization Rates

Objective: To assess the relationships among human follicular fluid-induced acrosome reaction,sperm morphology and in vitro fertilization rates.Methods: The relationships among human follicular fluid-induced acrosome reaction,sperm morphology and in vitro fertilization rates were investigated by Spearman rank correlation in 79 infertile couples.And the sperm morphology analysis was performed by crystal violet staining and based on strict criteria.Results: A significant positive correlation was found between the percentage of human follicular fluid-induced acrosome reaction and that of normal sperm morphology(n=49,r=(0.376 3),P<(0.01)),but no significant correlation was observed either between the percentage of human follicular fluid-induced acrosome reaction and in vitro fertilization rates or between that of normal sperm morphology and in vitro fertilization rates(n=21,r=(0.266 6),P>(0.05) and n=50,r=(0.001 8),P>(0.05),respectively).Conclusion: There is a significant positive correlation between the percentage of human follicular fluid-induced acrosome reaction and that of normal sperm morphology,but no such correlation either between the percentage of human follicular fluid-induced acrosome reaction and in vitro fertilization rates or between that of normal sperm morphology and in vitro fertilization rates.