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In situ detection of antigen-specific tumor-infiltrating lymphocytes using newly designed tetramers |
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| Authors: | Bras-Gonçalves Rachel Le Gal Frédérique-Anne Avril Marie-Françoise Dalod Marc Connan Francine Chaboissier Aude Choppin Jeannine Guillet Jean-Gérard |
| Institution: | a Département d'Immunologie, Inserm U567,CNRS UMR 8104, Université Paris V René Descartes, IFR Alfred Jost, Institut Cochin, 27 rue du Faubourg Saint Jacques 75014, Paris, France b Service de Dermatologie, Institut Gustave Roussy, 39, rue Camille-Desmoulins, F-94805, Villejuif Cedex, France |
| Abstract: | We described a new process for the design of HLA tetramers using soluble MHC class I molecules purified from Epstein Barr Virus-transformed B cells. This method does not rely on genetic engineering and presents a significant advantage in view of the polymorphism of MHC class I molecules because tetramers can be produced with any HLA molecule. Here, we showed that our HLA-A*0201 tetramers provided experimental results similar to those obtained with tetramers made with recombinant MHC molecules. Moreover, they can be used to efficiently identify peptide-specific T cells from ex vivo PBMCs as well as from lymphocytes infiltrating human tumor. This innovative and simple method could be widely adopted, specially in diagnostic procedures for monitoring peptide-based immunotherapy. |
| Keywords: | Human leukocyte antigen (HLA) HLA-A*0201 tetramers In situ tetramer staining CD8+ T lymphocytes Tumor-infiltrating lymphocytes (TILs) |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |