复方861对肝星状细胞的增殖和凋亡的干预作用

目的 研究ＨＳＣ在体外和体内的增殖和凋亡及中药的干预作用。方法 体外研究对象为ＨＳＣ系。结果增殖采用ＭＴＴ比色法，细胞凋亡采用电镜观察，流式细胞仪和ＴＵＮＥＬ法检测。临床研究对象是量慢性乙型肝炎患者。结果 复方８６１显著抑制体外培养的ＨＳＣ增殖，随着药物剂量的增加和时间的延长，ＨＳＣ的凋亡明显增多，凋亡率增加，呈剂量和时间依赖，ＴＵＮＥＬ法检测，用５ｍｇ／ｍｌ复方８６１作用４８ｈ二，ＨＳＣ凋亡率为

Proliferationand apoptosis of hepatic stellate cells and effects of compound 861 on liver fibrosis

Objective To investigate the proliferation and apoptosis of the hepatic stellate cell (HSC) in vitro and in vivo and the effects of Chinese herb, compound 861. Methods The in vitro study was carried out on the culture of hepatic stellate cell line. Various concentrations of compound 861 were added and incubated. Cell proliferation was detected with MTT colorimetric assay. Cell apoptosis was detected by electron microscopy, flow cytometry and TUNEL. The subjects of in vivo study were patients with chronic hepatitis B. Results Compound 861 could significantly inhibit HSC proliferation and increase the apoptosis rate of HSC dose-dependently and time-dependently compared with the control group. After compound 861 incubation for 48h, the apoptosis rate of HSC was 25.9% compared with 9.2% in control group(p<0.05). The clinical study elucidated that the HSC was activated and proliferated in patients with chronic hepatitis B. After compound 861 treatment for 6 months, the number of activated HSC decreased and the apoptosis of HSC could be seen in the liver biopsy. Conclusion Apoptosis of activated HSC exists in vitro and in vivo, stimulating its apoptosis may play an important role in the treatment of liver fibrosis.