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Dopamine-stimulated adenylate cyclase and tyrosine hydroxylase in diabetic rat retina
Authors:Frances K. Northington   Robert W. Hamill  Shailesh P. Banerjee  
Affiliation:1. Department of Pharmacology, Monroe Community Hospital, Rochester, NY ,U.S.A.;2. Neurology and Medicine, Monroe Community Hospital, Rochester, NY ,U.S.A.;3. Center for Brain Research, University of Rochester School of Medicine and Dentistry, Monroe Community Hospital, Rochester, NY ,U.S.A.;4. The Neurology Unit, Monroe Community Hospital, Rochester, NY ,U.S.A.;2. FAST BioMedical, Indianapolis, Indiana;1. Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia;2. Department of Cellular and Molecular Physiology, College of Medicine, Pennsylvania State University, Hershey, PA 17033, United States;1. Laboratory of Neurobiology and Experimental Neurology, Department of Physiology Faculty of Medicine, University of La Laguna, La Laguna, Tenerife, Canary Islands, Spain;2. Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Madrid, Spain;1. AMB, UiT The Arctic University of Norway, N-9037 Tromsø, Norway;2. NFH, UiT The Arctic University of Norway, N-9037 Tromsø, Norway;3. Marbio, UiT The Arctic University of Norway, N-9037 Tromsø, Norway;1. Research Imaging Centre, Centre for Addiction and Mental Health, 250 College Street, Toronto, Ontario, M5T 1R8, Canada;2. Department of Psychiatry, University of Toronto, 250 College Street, Toronto, Ontario, M5T 1R8, Canada;3. Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, 250 College Street, Toronto, Ontario, M5T 1R8, Canada;4. Molecular and Cellular Cognition Lab, German Center for Neurodegenerative Diseases(DZNE), Bonn, Germany;5. Department of Pharmacology and Toxicology, University of Toronto, 250 College Street, Toronto, Ontario, M5T 1R8, Canada;6. Retina Department, Clínica David-Unidad Oftalmológica, Morelia, Mexico
Abstract:The effects of streptozotocin-induced diabetes on the retinal dopaminergic system have been examined in Long-Evans (pigmented) rats. Tyrosine hydroxylase activity was significantly decreased while dopamine-stimulated adenylate cyclase was increased in 2-month-diabetic rats. The observed increase in dopamine-stimulated adenylate cyclase activity in diabetic retinae may be related to neurotransmitter receptor changes because postreceptor activation of adenylate cyclase by guanylyl imidodiphosphate was not altered.
Keywords:diabetes   retina   tyrosine hydroxylase   adenylate cyclase   dopamine
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