| 滋补肝肾方含药血清对B16黑素细胞活性黑素含量及cAMP水平的影响 |
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| 引用本文： | 张蕾,赵京霞,李萍,刘卫红,王莒生.滋补肝肾方含药血清对B16黑素细胞活性黑素含量及cAMP水平的影响[J].辽宁中医杂志,2009(3):467-469. |
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| 作者姓名： | 张蕾 赵京霞 李萍 刘卫红 王莒生 |
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| 作者单位： | 首都医科大学附属北京中医医院北京市中医研究所;首都医科大学附属北京中医医院; |
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| 基金项目： | 国家自然科学基金资助(30572396);;北京市自然科学基金资助(7062011) |
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| 摘 要： | 目的:通过对含滋补肝肾中药大鼠血清对黑素细胞(B16)活性、黑素含量和cAMP水平影响的研究,探讨滋补肝肾方治疗白癜风的机制。方法:体外培养小鼠B16细胞,于对数生长期加不同浓度含滋补肝肾中药的大鼠血清培养后,分别采用MTT法、NaOH法和放免法检测细胞活性、黑素含量和cAMP水平。结果:与空白对照组比较,滋补肝肾方含药血清各剂量组对B16增殖均没有明显促增殖活性;但20%含药血清可明显促进B16合成黑素量(P<0.05),并提高细胞内cAMP水平(P<0.05)。结论:滋补肝肾方可能通过提高黑素细胞cAMP的水平,从而促进B16细胞合成黑素的功能。
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| 关 键 词： | 滋补肝肾方 含药血清 黑素合成 cAMP |
In vitro Study of Drug-contained Serum with Chinese Medicines Nourish Liver and Kidney on B16 Melanocyte Cytotoxicity,Melanin Synthesis and cAMP |
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| ZHANG Lei,ZHAO Jing-xia,LI Ping,LIU Wei-hong,WANG Ju-sheng.In vitro Study of Drug-contained Serum with Chinese Medicines Nourish Liver and Kidney on B16 Melanocyte Cytotoxicity,Melanin Synthesis and cAMP[J].Liaoning Journal of Traditional Chinese Medicine,2009(3):467-469. |
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| Authors: | ZHANG Lei ZHAO Jing-xia LI Ping LIU Wei-hong WANG Ju-sheng |
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| Institution: | 1.Beijing Institute of Traditional Chinese Medicine;Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital University of Medical Science;Beijing 100010;China;2.Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital University of Medical Science;China |
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| Abstract: | Objective:To study effects of drug-contained serum with Chinese Medicines nourish liver and kidney on melanocytes(B16) cytotoxicity and melanin synthesis and cAMP;explore the possible roles of nourish liver and kidney decoction in vitiligo treatment.Methods:Mice B16 cells were cultivated in vitro.Different concentrations rat of drug-contained serum were added to the culture medium during logarithmic growth phase.Cytotoxicity,melanin and cAMP were measured by MTT assay,NaOH assay and radioimmunoassay respect... |
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| Keywords: | Nourish Liver and Kidney Drug-contained Serum Melanin synthesis cAMP |
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