视神经脊髓炎患者水通道蛋白4抗体的临床观察

目的：观察水通道蛋白4（AQP4）抗体在视神经脊髓炎（NMO）、多发性硬化（MS）及其他神经系统疾病中的灵敏度和特异性。方法用间接免疫荧光法检测90例NMO、MS及其他神经系统疾病患者AQP4抗体,探讨AQP4抗体的诊断及鉴别诊断价值。结果视神经脊髓炎患者中AQP4抗体阳性70%,特异性达91%。AQP4滴度在NMO, MS及其他神经科疾病患者中差异有统计学意义（P〈0.05）。结论 AQP4抗体在诊断、鉴别诊断NMO, MS及其他神经系统疾病中有很高的应用价值。

Correlation of DNA content,DNA ploidy status and lymph node metastasis of esophageal squamous cell carcinoma

Objective To investigate the correlation of DNA content, DNA ploidy status and lymph node metastasis of esophageal squamous cell carcinoma（ESCC）. Methods DNA analysis was carried out respectively by flow cytometry in the specimens from 167 patients with resectable ESCC. DNA index （DI） and ploidy status was measured respectively. Then analyze the correlation of DI and DNA ploidy status with lymph node metastasis of ESCC. Results ①Heteroploidy rate in ESCC was 81.44%. Even in early stage of ESCC, the diversity of DNA index and ploidy status could be detected; ②DNA index was higher in group with lymph node metastasis than that without lymph node metastasis;③Comparing with ESCC with DNA diploidy, ESCC with DNA heteroploidy showed higher lymph nodes metastasis rate and lymph node metastasis ratio. Lymph node metastasis occurred even in early stage of ESCC with DNA heteroploidy. Conclusion Esophageal squamous cell carcinoma with higher DNA content or DNA heteroploidy more likely metastasizes to lymph node.