首页 | 本学科首页   官方微博 | 高级检索  
     


Inhibition of autophagy by geniposide protects against myocardial ischemia/reperfusion injury
Affiliation:1. Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China;2. Department of Vascular Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China;1. Department of Cardiology, Heart Center, Zhujiang Hospital of Southern Medical University, NO. 253, Gongye Avenue, 510282, Guangzhou, China;2. Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, No. 1, East Jianshe Road, 450052, Zhengzhou, China;1. Central Laboratory, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China;2. Beijing University of Chinese Medicine, Beijing, China;3. Research Department, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China;1. Department of Medical Affairs, No.1 People’s Hospital of Jining City, Jining, Shandong, China;2. Department of Emergency, No.1 People’s Hospital of Jining City, Jining, Shandong, China;1. Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, PR China;2. Hubei Key Laboratory of Metabolic and Chronic Diseases, Wuhan, 430060, PR China
Abstract:Geniposide (GP), extracted from a traditional Chinese herb Gardenia jasminoides, has extensive pharmacological effects. But the effects and the potential mechanisms of GP on myocardial ischemia/reperfusion (I/R) injury are poorly understood. In present study, we investigated the effect of GP on myocardial I/R injury in vivo and hypoxia/reoxygenation (H/R) in vitro respectively, and its mechanism. The results showed that GP reduced myocardial infarct size, alleviated acute myocardial injury, improved cardiac function, regulated apoptosis-related proteins and inhibited apoptosis. In vitro experiments revealed that GP enhanced the cell viability, regulated apoptosis-related proteins and prevented cell apoptosis during H/R in H9c2 cells. GP inhibited the expression of autophagy-related proteins and autophagosome accumulation both in vivo and in vitro. The effects of GP were blocked by rapamycin (RAPA) administration. In summary, our results showed that GP protected against myocardial I/R injury and involved inhibition of autophagy, which might be through activating AKT/mTOR signaling pathways.
Keywords:Geniposide  Autophagy  Apoptosis  Hypoxia/reoxygenation  Ischemia/reperfusion
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号