Aspirin enhances regulatory functional activities of monocytes and downregulates CD16 and CD40 expression in myocardial infarction autoinflammatory disease |
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Affiliation: | 1. Laboratory of Applied Molecular Biology and Immunology, BioMolIm, W0414100, University of Tlemcen, 13000 Tlemcen, Algeria;2. EHU “1er Novembre 1954” - Hemobiology, Oran, Algeria;3. Department of Biotechnology, University of Oran, 1 Ahmed Ben Bella, 31000 Oran, Algeria |
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Abstract: | BackgroundExacerbation of CD16 as molecule marker of both intermediate and non-classical monocytes (MOs) has been shown to be involved in the pathogenesis of myocardial infarction (MI). In this study, we have tried to evaluate the aspirin (acetylsalicylic acid, ASA) treatment effect on the CD16-expressed MOs and activation-associated CD40 in MI.MethodsMOs were isolated from the whole blood of healthy controls and patients with MI. The cells were stimulated and treated with different doses of ASA.ResultsASA significantly decreased nitric oxide (NO) production and inducible NO synthase (iNOS) activity, but significantly increased arginase activity. Levels of interleukin (IL)-1β, IL-6 and interferon-γ (IFN-γ) were downregulated, whereas those of IL-10 were upregulated. Additionally, ASA induced a markedly increase in both phagocytosis and intracellular pathogen killing activities. Moreover, ASA treatment induced significantly upregulation of intracellular levels of glucose (iGlu), and free calcium ions (ifCa2+), and, covertly, significantly downregulation of total cellular cholesterol content (tccCHOL). Furthermore, the expression levels of CD16 and CD40 were significantly downregulated in ASA-treated MOs.ConclusionsWe show for the first time that ASA immunomodulates the functional activities of MOs during MI and promotes their switching toward a classical phenotype, exhibiting low CD16 expression levels and thereby anti-inflammatory properties. |
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Keywords: | Aspirin Human monocyte CD16 (FcRγIIIa) and CD40 expression Immunomodulation Monocyte phenotypes and functional activities Myocardial infarction autoinflammatory disease |
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